Human complement component 4 binding protein, beta ELISA Kit | C4BPB elisa kit

Human C4b-binding protein beta chain, C4BPB ELISA Kit

Cat. Num. AAA919306

• Gene Names: C4BPB; C4BP
• Reactivity: Human
• Synonyms: complement component 4 binding protein; beta; Human C4b-binding protein beta chain; C4BPB ELISA Kit; Human C4b-binding protein beta chain (C4BPB) ELISA kit; C4BP; C4b binding protein; beta chain; complement component 4-binding protein; C4BPB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human C4BPB. No significant cross-reactivity or interference between human C4BPB and analogues was observed.
• Sequence Length: 252

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 3.12 ng/ml -200 ng/ml.
• Sensitivity: The minimum detectable dose of human C4BPB is typically less than 0.78 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C4BPB elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C4BPB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C4BPB present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for C4BPB is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C4BPB bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4502505
• NCBI GeneID: 725
• NCBI Accession #: NP_000707.1
• NCBI GenBank Nucleotide #: NM_000716.3
• UniProt Accession #: P20851; Q5VVR0; Q9BS25; A5JYP8; D3DT81
• Molecular Weight: 28,286 Da
• NCBI Official Full Name: C4b-binding protein beta chain isoform 1
• NCBI Official Synonym Full Names: complement component 4 binding protein, beta
• NCBI Official Symbol: C4BPB
• NCBI Official Synonym Symbols: C4BP
• NCBI Protein Information: C4b-binding protein beta chain
• UniProt Protein Name: C4b-binding protein beta chain
• Protein Family: C4b-binding protein
• UniProt Gene Name: C4BPB
• UniProt Entry Name: C4BPB_HUMAN

NCBI Description

This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. A single, unique beta-chain encoded by this gene assembles with seven identical alpha-chains into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. C4b-binding protein has a regulatory role in the coagulation system also, mediated through the beta-chain binding of protein S, a vitamin K-dependent protein that serves as a cofactor of activated protein C. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Alternative splicing gives rise to multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

C4BPB: Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: protein binding

Biological Process: negative regulation of complement activation, classical pathway; positive regulation of protein catabolic process; regulation of complement activation; innate immune response; blood coagulation; complement activation, classical pathway