Mouse filaggrin ELISA Kit | FLG elisa kit

Mouse Filaggrin, FLG ELISA Kit

Cat. Num. AAA940555

• Reactivity: Mouse
• Synonyms: filaggrin; Mouse Filaggrin; FLG ELISA Kit; Mouse Filaggrin (FLG) ELISA kit; ATOD2; epidermal filaggrin; FLG elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse FLG. No significant cross-reactivity or interference between mouse FLG and analogues was observed.
• Sequence Length: 336

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 3.12 pg/ml-200 pg/ml.
• Sensitivity: The minimum detectable dose of mouse FLG is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLG elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FLG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FLG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FLG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FLG bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 120164
• UniProt Accession #: P11088
• Molecular Weight: 35,678 Da
• NCBI Official Full Name: Filaggrin, partial
• UniProt Protein Name: Filaggrin
• Protein Family: Filaggrin
• UniProt Gene Name: Flg
• UniProt Entry Name: FILA_MOUSE

Uniprot Description

FLG: Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. Defects in FLG are the cause of ichthyosis vulgaris (VI); also known as ichthyosis simplex. Ichthyosis vulgaris is the most common form of ichthyosis inherited as an autosomal dominant trait. It is characterized by palmar hyperlinearity, keratosis pilaris and a fine scale that is most prominent over the lower abdomen, arms, and legs. Ichthyosis vulgaris is characterized histologically by absent or reduced keratohyalin granules in the epidermis and mild hyperkeratosis. The disease can be associated with frequent asthma, eczema or hay fever. Defects in FLG are a cause of susceptibility to dermatitis atopic type 2 (ATOD2). Atopic dermatitis is a complex, inflammatory disease with multiple alleles at several loci thought to be involved in the pathogenesis. It commonly begins in infancy or early childhood and is characterized by a chronic relapsing form of skin inflammation, a disturbance of epidermal barrier function that culminates in dry skin, and IgE- mediated sensitization to food and environmental allergens. It is manifested by lichenification, excoriation, and crusting, mainly on the flexural surfaces of the elbow and knee. Belongs to the S100-fused protein family.

Protein type: Cytoskeletal

Cellular Component: cornified envelope

Molecular Function: structural constituent of epidermis

Biological Process: epidermis development; multicellular organismal development; intermediate filament organization