Mouse fibulin 1 ELISA Kit | FBLN1 elisa kit

Mouse Fibulin-1, FBLN1 ELISA Kit

Cat. Num. AAA923854

• Reactivity: Mouse
• Synonyms: fibulin 1; Mouse Fibulin-1; FBLN1 ELISA Kit; Mouse Fibulin-1 (FBLN1) ELISA kit; CTA-941F9.7; FBLN; ; FBLN1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse FBLN1. No significant cross-reactivity or interference between mouse FBLN1 and analogues was observed.
• Sequence Length: 705

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 3.12 ng/ml -200 ng/ml.
• Sensitivity: The minimum detectable dose of mouse FBLN1 is typically less than 0.78 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBLN1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FBLN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FBLN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FBLN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FBLN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 168693628
• NCBI GeneID: 14114
• NCBI Accession #: NP_034310.2
• NCBI GenBank Nucleotide #: NM_010180.2
• UniProt Accession #: Q08879; Q08878; Q8C3B1; Q91ZC9; Q922K8
• Molecular Weight: 78,033 Da
• NCBI Official Full Name: fibulin-1
• NCBI Official Synonym Full Names: fibulin 1
• NCBI Official Symbol: Fbln1
• NCBI Protein Information: fibulin-1; BM-90; FIBL-1; basement-membrane protein 90; BM-90/fibulin extracellular matrix glyroprotein
• UniProt Protein Name: Fibulin-1
• UniProt Gene Name: Fbln1
• UniProt Synonym Gene Names: FIBL-1; BM-90
• UniProt Entry Name: FBLN1_MOUSE

Uniprot Description

FBLN1: Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP. A chromosomal aberration involving FBLN1 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. Fibroblasts derived from a patient with synpolydactyly displayed alterations in the level of isoform D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of isoform C was not perturbed in the patients fibroblasts. Furthermore, no aberrant polypeptides were detected in extracts of cultured patients fibroblasts. The translocation t(12;22) may result in haploinsufficiency of the isoform D splice variant, which could lead to the observed limb malformation. Elevated expression and altered processing of FBLN1 protein is associated with human breast cancer. Belongs to the fibulin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Extracellular matrix; Secreted, signal peptide

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region; basement membrane

Molecular Function: protein binding; protease activator activity; calcium ion binding

Biological Process: extracellular matrix organization and biogenesis