Typical Testing Data/Standard Curve (for reference only)
Mouse IL-17D ELISA Kit | IL17D elisa kit
Mouse IL-17D ELISA Kit
Reactivity
Mouse
Applications
ELISA
Synonyms
IL-17D; Mouse IL-17D ELISA Kit; IL27; Interleukin-17D; Interleukin-27; IL-27; IL17D elisa kit
Reactivity
Mouse
Specificity
The Mouse IL-17D ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse IL-17D proteins within the range of 62.5 pg/ml - 4000 pg/ml.
Sequence Length
205
Applicable Applications for IL17D elisa kit
Sandwich ELISA (EIA)
Samples
Cell Culture Supernatants, Serum, Plasma
Sensitivity
10 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL17D elisa kit
Principle of the Assay: The Mouse IL-17D ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-17D in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse IL-17D coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17D present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-17D antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17D bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Interleukin 17D (IL-17D) is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines. And it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas.
Background/Introduction: Interleukin 17D (IL-17D) is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines. And it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
22,391 Da
NCBI Official Full Name
IL-17D
UniProt Protein Name
IL-17D
Protein Family
UniProt Gene Name
Il17d
UniProt Entry Name
Q8K4C4_MOUSE
Uniprot Description
IL17D: Induces expression of IL-6, IL-8, and GM-CSF from endothelial cells. Belongs to the IL-17 family.
Protein type: Secreted, signal peptide; Cytokine; Secreted
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Product Notes
The Mouse IL17D il17d (Catalog #AAA8244599) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8244599 ELISA Kit recognizes Mouse IL17D. AAA Biotech's IL-17D can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the IL17D il17d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-17D, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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