Typical Testing Data/Standard Curve (for reference only)
Mouse MMP12 ELISA Kit | MMP12 elisa kit
Mouse MMP12 ELISA Kit
Gene Names
Mmp12; Mmel; AV378681
Reactivity
Mouse
Applications
ELISA
Synonyms
MMP12; Mouse MMP12 ELISA Kit; HME; Macrophage metalloelastase; MME; Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12; MMP12 elisa kit
Reactivity
Mouse
Specificity
The Mouse MMP12 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse MMP12 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
Sequence Length
473
Applicable Applications for MMP12 elisa kit
Sandwich ELISA (SE)
Samples
Cell Culture Supernatants, Serum, Plasma
Sensitivity
10 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MMP12 elisa kit
Principle of the Assay: Mouse MMP12 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse MMP12 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse MMP12 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP12 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse MMP12 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP12 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Matrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.
Background/Introduction: Matrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,971 Da
NCBI Official Full Name
macrophage metalloelastase
NCBI Official Synonym Full Names
matrix metallopeptidase 12
NCBI Official Symbol
Mmp12
NCBI Official Synonym Symbols
Mmel; AV378681
NCBI Protein Information
macrophage metalloelastase; MME; MMP-12; macrophage elastase; macrophage-metalloelastase; matrix metalloproteinase 12; matrix metalloproteinase-12
UniProt Protein Name
Macrophage metalloelastase
Protein Family
UniProt Gene Name
Mmp12
UniProt Synonym Gene Names
Mme; Mmel; MME; MMP-12
UniProt Entry Name
MMP12_MOUSE
Uniprot Description
MMP12: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Belongs to the peptidase M10A family.
Protein type: EC 3.4.24.65; Secreted, signal peptide; Secreted; Protease
Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular region
Molecular Function: peptidase activity; zinc ion binding; metallopeptidase activity; hydrolase activity; metalloendopeptidase activity; metal ion binding; calcium ion binding
Biological Process: wound healing; positive regulation of epithelial cell proliferation involved in wound healing; proteolysis; wound healing, spreading of epidermal cells
Research Articles on MMP12
Similar Products
Product Notes
The Mouse MMP12 mmp12 (Catalog #AAA824765) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA824765 ELISA Kit recognizes Mouse MMP12. AAA Biotech's MMP12 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (SE). Researchers should empirically determine the suitability of the MMP12 mmp12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP12, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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