Rat IL-1 beta ELISA Kit | IL1B elisa kit

Rat IL-1 beta ELISA Kit

Cat. Num. AAA824956

• Reactivity: Rat
• Applications: ELISA
• Synonyms: IL-1 beta; Rat IL-1 beta ELISA Kit; IL1F2; Interleukin-1 beta; Catabolin; IL1B elisa kit

• Reactivity: Rat
• Specificity: The Rat IGF2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat IGF2 proteins within the range of 62.5 pg/ml-4000 pg/ml.
• Sequence Length: 268

• Applicable Applications for IL1B elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma
• Sensitivity: 5 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL1B elisa kit

Principle of the Assay: MBS Rat IGF2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IGF2 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat IGF2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IGF2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.

NCBI and Uniprot Product Information

• NCBI GI #: 2497335
• NCBI GeneID: 24494
• UniProt Accession #: Q63264
• Molecular Weight: 30,644 Da
• NCBI Official Full Name: Interleukin-1 beta
• NCBI Official Synonym Full Names: interleukin 1 beta
• NCBI Official Symbol: Il1b
• NCBI Protein Information: interleukin-1 beta; IL-1 beta
• UniProt Protein Name: Interleukin-1 beta
• Protein Family: Interleukin
• UniProt Gene Name: Il1b
• UniProt Synonym Gene Names: IL-1 beta
• UniProt Entry Name: IL1B_RAT

NCBI Description

an inflammatory cytokine [RGD, Feb 2006]

Uniprot Description

IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family.

Protein type: Cytokine

Cellular Component: extracellular space; extracellular region; vesicle; secretory granule

Molecular Function: protein domain specific binding; interleukin-1 receptor binding; cytokine activity

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of JNK activity; negative regulation of MAP kinase activity; positive regulation of nitric oxide biosynthetic process; glycoprotein metabolic process; negative regulation of glutamate secretion; positive regulation of apoptosis; activation of MAPK activity; positive regulation of interleukin-2 biosynthetic process; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; germ cell programmed cell death; negative regulation of insulin receptor signaling pathway; response to lipopolysaccharide; positive regulation of glial cell differentiation; positive regulation of NF-kappaB import into nucleus; positive regulation of lipid catabolic process; fever; response to organic cyclic substance; positive regulation of membrane protein ectodomain proteolysis; response to carbohydrate stimulus; activation of NF-kappaB transcription factor; elevation of cytosolic calcium ion concentration; response to vitamin D; pentacyclic triterpenoid metabolic process; positive regulation of phagocytosis; positive regulation of T cell proliferation; positive regulation of astrocyte differentiation; response to drug; neutrophil chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of heterotypic cell-cell adhesion; positive regulation of mitosis; interleukin-1 beta production; positive regulation of interleukin-6 production; social behavior; response to organic nitrogen; purine base metabolic process; negative regulation of neuron differentiation; positive regulation of angiogenesis; response to ethanol; response to heat; positive regulation of cell division; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of lipid metabolic process; leukocyte migration; estrogen metabolic process; response to peptide hormone stimulus; sequestering of triacylglycerol; wound healing; positive regulation of interleukin-6 biosynthetic process; response to morphine; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; response to L-ascorbic acid; chronic inflammatory response to antigenic stimulus; response to estradiol stimulus; positive regulation of stress-activated MAPK cascade; negative regulation of neurogenesis; positive regulation of interleukin-8 production; negative regulation of cell proliferation; learning and/or memory; negative regulation of lipid catabolic process; hyaluronan biosynthetic process; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; response to gamma radiation; inflammatory response; regulation of I-kappaB kinase/NF-kappaB cascade; response to nutrient; aging; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; positive regulation of immature T cell proliferation in the thymus; response to ATP; memory; ovulation; polyketide metabolic process; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; response to ozone; positive regulation of prostaglandin secretion; positive regulation of fever; response to hypoxia; immune response; positive regulation of protein amino acid phosphorylation; regulation of insulin secretion