Rat IL-4 ELISA Kit | IL4 elisa kit

Rat IL-4 ELISA Kit

Cat. Num. AAA824713

• Gene Names: Il4; Il4e12
• Reactivity: Rat
• Applications: ELISA
• Synonyms: IL-4; Rat IL-4 ELISA Kit; Interleukin-4; B-cell stimulatory factor 1; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra; IL4 elisa kit

• Reactivity: Rat
• Specificity: The Rat IL-1 beta ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat IL-1 beta proteins within the range of 62.5 pg/ml-4000 pg/ml.
• Sequence Length: 147

• Applicable Applications for IL4 elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates
• Sensitivity: 15 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL4 elisa kit

Principle of the Assay: MBS Rat IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-1 beta in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Rat IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

NCBI and Uniprot Product Information

• NCBI GI #: 42627877
• NCBI GeneID: 287287
• NCBI Accession #: NP_958427.1
• NCBI GenBank Nucleotide #: NM_201270.1
• UniProt Accession #: P20096
• Molecular Weight: 16,247 Da
• NCBI Official Full Name: interleukin-4
• NCBI Official Synonym Full Names: interleukin 4
• NCBI Official Symbol: Il4
• NCBI Official Synonym Symbols: Il4e12
• NCBI Protein Information: interleukin-4; IL-4; BSF-1; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IGG differentiation factor
• UniProt Protein Name: Interleukin-4
• Protein Family: Interleukin
• UniProt Gene Name: Il4
• UniProt Synonym Gene Names: Il-4; IL-4; BSF-1
• UniProt Entry Name: IL4_RAT

NCBI Description

Th2-type cytokine; may be involved in inflammatory response in eosinophils [RGD, Feb 2006]

Uniprot Description

IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cytokine; Secreted, signal peptide; Secreted; Cell cycle regulation

Cellular Component: extracellular space; external side of plasma membrane

Molecular Function: growth factor activity; cytokine activity; interleukin-4 receptor binding

Biological Process: negative regulation of osteoclast differentiation; positive regulation of isotype switching to IgG isotypes; positive regulation of transcription, DNA-dependent; microglial cell activation; regulation of proton transport; female pregnancy; positive regulation of activated T cell proliferation; response to organic cyclic substance; positive regulation of interleukin-10 production; positive regulation of isotype switching to IgE isotypes; B cell costimulation; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; response to nutrient; response to drug; cholesterol metabolic process; regulation of immune response; negative regulation of chronic inflammatory response; B cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; positive regulation of mast cell degranulation; defense response to protozoan; positive regulation of immunoglobulin production; T-helper 1 cell lineage commitment; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; positive regulation of chemokine biosynthetic process; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of T cell differentiation; innate immune response in mucosa; retina development in camera-type eye; positive regulation of B cell activation; response to cytokine stimulus; regulation of inflammatory response; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; T-helper 2 cell differentiation; positive regulation of defense response to virus by host; negative regulation of transcription, DNA-dependent; negative regulation of T cell activation; regulation of T cell activation