Interleukin-2 ELISA Kit | IL-2 sRa elisa kit

Interleukin-2 Soluble Receptor alpha (IL-2 sRa)

Cat. Num. AAA590008

• Gene Names: IL2RA; p55; CD25; IL2R; TCGFR; IDDM10
• Synonyms: Interleukin-2; Interleukin-2 Soluble Receptor alpha (IL-2 sRa); IL-2 sRa elisa kit

• Specificity: This sandwich ELISA can detect both natural and recombinant human IL-2 sRalpha. This kit exhibits no significant cross-reactivity with factors related to or associated with IL-2 sRalpha such as rhIL-2, rhIL-2 sRalpha. No significant cross-reactivity was observed with recombinant human: IL-1alpha, IL-1beta, IL-1 sRI, IL-1 sRII, IL-1ra, IL-3, IL-3 sR alpha, IL-5, IL-5 sR alpha, IL-5 sRb, IL-6, IL-6 sR, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13 ANG, AR, CNTF, beta-ECGR, EGF, EPO, FGF acidic, FGF basic, FGF-4, FGF-5, FGF-6, FGF-7, GCSF, GM-CSF, sgp 130, GRO alpha, GRObeta, GROgamma, HB-EGF, HGF, INF-gamma, IGF-I IGF-II, LAP (TGF-beta1), LIF, M-CSF, MCP-1, MIP-1alpha, MIP-1beta, beta-NGF, OSN, PD-ECGF, PDGFAA, PDGF-AB, PDGF-BB, PTN, RANTES, SCF, SLPI, TGF- alpha, TGF-beta, TNF- alpha, TNF- beta, sTNF RI, sTNF RII, VEGF.
• Sequence Length: 272

• Samples: Serum, plasma, Cell Culture Supernatant, and other biological fluids
• Assay Type: Sandwich
• Sensitivity: The minimum detectable quantity of human IL-2 sRalpha as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II is 10 pg/mL. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities.
• Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by replicates of twenty in 1 assay.
• Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by replicates on 20 different assays.
• Intended Uses: This Human Interleukin-2 soluble receptor alpha ELISA Kit is to be used for the in vitro quantitative determination of human interleukin-2 soluble receptor alpha (IL-2 sRalpha) concentrations in serum, plasma, cell culture supernatant, and other biological fluids. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not to be used in therapeutic procedures.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-2 sRa elisa kit

Principle of the assay: This IL-2 sRalpha enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-2 sRalpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-2 sRalpha and incubated. IL-2 sRalpha, if present, will bind and become immobilized by the antibody pre-coated on the wells and then become "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL- 2 sRalpha and other components of the sample. In order to quantitatively determine the amount of IL-2sRalpha present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-2 sRalpha, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm +/- 2nm. In order to measure the concentration of IL-2 sRalpha in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-2 sRalpha concentration (pg/mL). The concentration of IL-2 sRalpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL-2 sRa elisa kit

ELISA Kits for Human Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4557667
• NCBI GeneID: 3559
• NCBI Accession #: NP_000408.1
• NCBI GenBank Nucleotide #: NM_000417.2
• Molecular Weight: 30,819 Da
• NCBI Official Full Name: interleukin-2 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 2 receptor, alpha
• NCBI Official Symbol: IL2RA
• NCBI Official Synonym Symbols: p55; CD25; IL2R; TCGFR; IDDM10
• NCBI Protein Information: interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; TAC antigen
• UniProt Protein Name: Interleukin-2 receptor subunit alpha
• Protein Family: Interleukin
• UniProt Gene Name: IL2RA
• UniProt Entry Name: IL2RA_HUMAN

NCBI Description

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

Uniprot Description

IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: interleukin-2 receptor activity; drug binding; interleukin-2 binding

Biological Process: Notch signaling pathway; regulation of T cell homeostatic proliferation; apoptosis; negative regulation of immune response; negative regulation of defense response to virus; positive regulation of activated T cell proliferation; cell proliferation; inflammatory response to antigenic stimulus; cell surface receptor linked signal transduction; negative regulation of T cell proliferation; positive regulation of T cell differentiation; negative regulation of inflammatory response; immune response; inflammatory response; activated T cell apoptosis

Disease: Interleukin 2 Receptor, Alpha, Deficiency Of; Diabetes Mellitus, Insulin-dependent, 10