Interleukin-5 ELISA Kit | IL-5 elisa kit

Interleukin-5 (IL-5)

Cat. Num. AAA590010

• Gene Names: IL5; EDF; TRF; IL-5
• Synonyms: Interleukin-5; Interleukin-5 (IL-5); IL-5 elisa kit

• Specificity: This sandwich ELISA can detect both natural and recombinant human IL-5. This kit exhibits no detectable cross-reactivity with human; GM-CSF, GRO a, GRO b, INF-g, INF a, IL-1a, IL-1b, IL-1 RA, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-9, IL-10, IL- 11, IL-12, IL-13, IL-15, TNF-a, mouse IL-5 and IL-9.
• Sequence Length: 134

• Samples: Serum, plasma, cell culture medium, and urine
• Assay Type: Sandwich
• Sensitivity: The minimum detectable quantities of human IL-5 as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 7.5 pg/mL and 6.3 pg/mL respectively. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities.
• Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by replicates of 20 in 1 assay.
• Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by replicates on 20 different assays.
• Intended Uses: This Human Interleukin 5 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 5 (IL-5) concentrations in serum, plasma, cell culture medium, and urine. This kit is intended for LABORATORY RESEARCH ONLY and is not to be used for therapeutic purposes.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-5 elisa kit

Principle of the assay: This IL-5 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for IL-5 and incubated. IL-5, if present, will bind and become immobilized by the antibody pre-coated on the wells and then become "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-5 and other components of the sample. In order to quantitatively determine the amount of IL-5 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-5, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm.

Product Categories/Family for IL-5 elisa kit

ELISA Kits for Human Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4504671
• NCBI GeneID: 3567
• NCBI Accession #: NP_000870.1
• NCBI GenBank Nucleotide #: NM_000879.2
• Molecular Weight: 15,238 Da
• NCBI Official Full Name: interleukin-5
• NCBI Official Synonym Full Names: interleukin 5
• NCBI Official Symbol: IL5
• NCBI Official Synonym Symbols: EDF; TRF; IL-5
• NCBI Protein Information: interleukin-5; B-cell differentiation factor I; T-cell replacing factor; colony-stimulating factor, eosinophil; eosinophil differentiation factor
• UniProt Protein Name: Interleukin-5
• Protein Family: Interleukin
• UniProt Gene Name: IL5
• UniProt Entry Name: IL5_HUMAN

NCBI Description

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Uniprot Description

IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; interleukin-5 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; immune response; positive regulation of transcription factor activity; positive regulation of immunoglobulin secretion; inflammatory response; positive regulation of eosinophil differentiation