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SDS-PAGE

IgG2b-Fc recombinant protein

Recombinant Mouse IgG2b-Fc Protein

Purity
> 92 % as determined by SDS-PAGE
Synonyms
IgG2b-Fc; Recombinant Mouse IgG2b-Fc Protein; Ig gamma-2 chain C region; IgG2B Fc; IgG2B-Fc; IgG2b-Fc recombinant protein
Ordering
For Research Use Only!
Host
HEK293 Cells
Purity/Purification
> 92 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
Sequence
Glu 97-Lys 335
Application Notes
The recombinant mouse IgG2b-Fc consists of 239 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, the apparent molecular mass of IgG2b-Fc is approximately 37 kDa in SDS-PAGE under reducing conditions.
Species
Mouse
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method.
Reconstitution
We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water(400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
Preparation and Storage
Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

SDS-PAGE
Related Product Information for IgG2b-Fc recombinant protein
A DNA sequence encoding the Mouse IgG2b Fc region (P01867-2) (Glu 97-Lys 335) was expressed and purified.

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
Mol_Mass: 26.3 kDa
AP_Mol_Mass: 37 kDa
NCBI Official Full Name
Ig gamma-2B chain C region
UniProt Protein Name
Ig gamma-2B chain C region
UniProt Gene Name
Igh-3

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Product Notes

The IgG2b-Fc igh-3 (Catalog #AAA2546663) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant mouse IgG2b-Fc consists of 239 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, the apparent molecular mass of IgG2b-Fc is approximately 37 kDa in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the IgG2b-Fc igh-3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Glu 97-Lys 335. It is sometimes possible for the material contained within the vial of "IgG2b-Fc, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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