TLR4 / CD284 recombinant protein

Recombinant Human TLR4 / CD284 Protein (His tag)

Cat. Num. AAA2546486

• Gene Names: TLR4; TOLL; CD284; TLR-4; ARMD10
• Purity: >87 % as determined by reducing SDS-PAGE.
• Synonyms: TLR4 / CD284; Recombinant Human TLR4 / CD284 Protein (His tag); ARMD10; CD284; TLR-4; TLR4; TOLL; TLR4 / CD284 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >87 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile 20mM PB, 500mM NaCl, 10% Glycerol, pH 6.0, 5%Trehalose, 5%Mannitol, 0.01% Tween-80
• Sequence Positions: Met 1-Lys631
• Sequence Length: 799

• Application Notes: The secreted recombinant human TLR4 consists of 619 amino acids and predicts a molecular mass of 70.5 KDa. The apparent molecular mass of the protein is approximately 68 Kda in SDS-PAGE under reducing conditions due to glycosylation.
• Species: Human
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water be added to the vial to prepare a stocksolution of 0.25 mg/mL. Concentration is measured by UV-Vis.
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Tag: C-His
• Preparation and Storage: Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4°C to 8°C for 2-7 days. Aliquots of reconstituted samples are stable at <-20 degree C for 3 months.

SDS-PAGE

Related Product Information for TLR4 / CD284 recombinant protein

TLR4, also known as TLR-4, is a member of the Toll-like receptor (TLR) family, which plays a fundamental role inpathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and sharestructural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressedon infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.TLR4 is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. TLR 4 has alsobeen designated as CD284 (cluster of differentiation 284). It has been implicated in signal transduction events induced bylipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 Cooperates with LY96 and CD14 to mediate theinnate immune response to bacterial lipopolysaccharide (LPS). It acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It is also involved in LPS-independentinflammatory responses triggered by Ni(2+).

NCBI and Uniprot Product Information

• NCBI GI #: 4507533
• NCBI GeneID: 7099
• NCBI Accession #: NP_003257.1
• NCBI GenBank Nucleotide #: NM_003266.3
• UniProt Accession #: O00206-1; Q5VZI8; Q5VZI9; Q9UK78; Q9UM57; A8K1Y8; A9XLP9; A9XLQ0; A9XLQ1; B4E194; D1CS52; D1CS53; O00206
• Molecular Weight: Mol_Mass: 70.5 kDa; AP_Mol_Mass: 68 kDa
• NCBI Official Full Name: toll-like receptor 4 isoform C
• NCBI Official Synonym Full Names: toll like receptor 4
• NCBI Official Symbol: TLR4
• NCBI Official Synonym Symbols: TOLL; CD284; TLR-4; ARMD10
• NCBI Protein Information: toll-like receptor 4
• UniProt Protein Name: Toll-like receptor 4
• UniProt Gene Name: TLR4

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

TLR4: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS- independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binding to bacterial LPS leads to homodimerization. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via their respective TIR domains. Interacts with NOX4. Interacts with CNPY3. Interacts with HSP90B1. The interaction with both CNPY3 and HSP90B1 is required for proper folding in the endoplasmic reticulum. Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. Belongs to the Toll-like receptor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 9q33.1

Cellular Component: cell surface; cytoplasm; endosome membrane; external side of plasma membrane; integral to plasma membrane; intrinsic to plasma membrane; lipopolysaccharide receptor complex; perinuclear region of cytoplasm; plasma membrane

Molecular Function: lipopolysaccharide binding; lipopolysaccharide receptor activity; protein binding; receptor activity

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; defense response to bacterium; defense response to Gram-negative bacterium; detection of lipopolysaccharide; I-kappaB kinase/NF-kappaB cascade; I-kappaB phosphorylation; immune response; inflammatory response; innate immune response; interleukin-1 beta secretion; lipopolysaccharide-mediated signaling pathway; macrophage activation; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of interferon-gamma production; negative regulation of interleukin-17 production; negative regulation of interleukin-23 production; negative regulation of interleukin-6 production; negative regulation of MyD88-independent toll-like receptor signaling pathway; negative regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of inflammatory response; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; positive regulation of interferon-gamma production; positive regulation of interleukin-1 production; positive regulation of interleukin-10 production; positive regulation of interleukin-12 biosynthetic process; positive regulation of interleukin-12 production; positive regulation of interleukin-6 production; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 production; positive regulation of NF-kappaB import into nucleus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of tumor necrosis factor production; response to lipopolysaccharide; toll-like receptor 4 signaling pathway; toll-like receptor signaling pathway

Disease: Macular Degeneration, Age-related, 10