cIAP1 / HIAP2 active protein

Recombinant Human cIAP1 / HIAP2 Protein (AVI tag)

Cat. Num. AAA2545921

• Gene Names: BIRC2; API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
• Purity: > 92 % as determined by reducing SDS-PAGE.
• Synonyms: cIAP1 / HIAP2; Recombinant Human cIAP1 / HIAP2 Protein (AVI tag); API1; c-IAP1; cIAP1; Hiap-2; HIAP2; MIHB; RNF48; cIAP1 / HIAP2 active protein

• Host: E.Coli
• Purity/Purification: > 92 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile 20mM Tris, 300mM NaCl, pH 8.0, 5%Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence Positions: Glu 144-Leu 356

• Species: Human
• Bio_activity: Measured by its ability to inhibit DEVD-AFC cleavage activity in cell extracts activated by addition of cytochrome c and dATP. The IC50 for this effect is typically 25-750 nM.
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex. ; It is recommended that sterile water(400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Ships on blue ice. Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

SDS-PAGE

Related Product Information for cIAP1 / HIAP2 active protein

The cellular inhibitor of apoptosis protein-1 (cIAP1) is a member of the Inhibitor of Apoptosis family proteinsare (IAP) whose members are characterized by a novel domain of about 70 amino acids termed baculoviral IAP repeats (BIRs). The BIR domains of cIAP1 and cIAP2 bind to caspases, the key effector proteases of apoptosis. The IAP protein family which can enhance cell survival are crucial regulators of programmed cell death. Both cIAP1 and cIAP2 are the E3 ubiquitin protein isopeptide ligases for Smac, taking part in promoting cancer survival through functioning as E3 ubiqitin ligases. Removal of cIAP1 by genetic deletion may result in NF-?B signaling activation that induces TNF? production and in killing sensitive tumor cells through enhanced TNF-R1 death-receptor signaling and caspase 8 activation. The substrate-dependent E3 activity of cIAPs is mediated by their RING domains and is dependent on the specific interactions between cIAPs and Smac. cIAP1 and cIAP2 are also reported to be regulators of NF-kB activation upon TNF α treatment.

NCBI and Uniprot Product Information

• NCBI GI #: 4502141
• NCBI GeneID: 329
• NCBI Accession #: NP_001157.1
• NCBI GenBank Nucleotide #: NM_001166.4
• UniProt Accession #: Q13490; Q16516; Q4TTG0; B4E026
• Molecular Weight: Mol_Mass: 26.5 kDa; AP_Mol_Mass: 26.5 kDa
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 2 isoform 1
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 2
• NCBI Official Symbol: BIRC2
• NCBI Official Synonym Symbols: API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
• NCBI Protein Information: baculoviral IAP repeat-containing protein 2
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 2
• UniProt Gene Name: BIRC2
• UniProt Synonym Gene Names: API1; MIHB; RNF48; hIAP-2; hIAP2

NCBI Description

The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

cIAP1: Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin- protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin- protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO and MXD1/MAD1. Can also function as an E3 ubiquitin- protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle. Interacts with DIABLO/SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. Interacts with CASP9. Interacts (via BIR domains) with TRAF2. Interacts with E2F1, RIPK1, RIPK2, RIPK3, RIPK4, BIRC5/survivin and USP19. Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes. The CARD domain inhibits the activation of E3 ubiquitin ligase activity by preventing RING domain dimerization and E2 ubiquitin donor binding and activation. The CARD domain- mediated autoinhibition of the E3 ubiquitin-protein ligase activity suppresses cell proliferation and migration. USP19 regulates the stability of BIRC2/c-IAP1 by preventing its ubiquitination. Belongs to the IAP family.

Protein type: EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 11q22.2

Cellular Component: cytoplasm; cytosol; internal side of plasma membrane; nucleus; spindle microtubule

Molecular Function: caspase inhibitor activity; chaperone binding; protein binding; protein N-terminus binding; transcription coactivator activity; transferase activity; ubiquitin binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: cell structure disassembly during apoptosis; cell surface receptor linked signal transduction; I-kappaB kinase/NF-kappaB cascade; negative regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; regulation of apoptosis; regulation of cell cycle; regulation of cell differentiation; regulation of cell proliferation; regulation of inflammatory response; regulation of innate immune response; regulation of toll-like receptor signaling pathway; tumor necrosis factor-mediated signaling pathway