Neurexin-3-beta / NRXN3 Active Protein | NRXN3 active protein

Recombinant Human NRXN3 Protein (His Tag)(Active)

Cat. Num. AAA2545650

• Gene Names: NRXN3; C14orf60
• Purity: > 94 % as determined by reducing SDS-PAGE.
• Synonyms: Neurexin-3-beta / NRXN3; Recombinant Human NRXN3 Protein (His Tag)(Active); C14orf60; KIAA0743; MGC176711; NRXN3 active protein

• Host: HEK293 Cells
• Purity/Purification: > 94 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.

• Application Notes: The recombinant human NRXN3 consists of 333 amino acids and predictes a molecular mass of 36 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhNRXN3 is approximately 50-60 kDa due to glycosylation.
• Predicted N Terminal: Ser 36
• Tag: C-His
• Bio_activity: Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to NRXN3 coated plates (0.8 ug/ml and 100 ul/well), approximately 30%-50% will adhere specifically after 60 minutes at 37 degree C.
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex.; It is recommended that sterile PBS, pH7.4 (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Ships on blue ice. Lyophilized proteins are stable for up to 12 months when stored at -20 degree C to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

SDS-PAGE

Related Product Information for NRXN3 active protein

Background: Neurexin-3-beta, also known as Neurexin III-beta and NRXN3, is a single-pass type I  membrane protein which belongs to the neurexin family. It contains one laminin G-like domain. It is a neuronal cell surface protein that may be involved in cell recognition and cell adhesion. Neurexins are a family of proteins that function in the vertebrate nervous system as cell adhesion molecules and receptors. They are encoded by several unlinked genes of which two, NRXN1 and NRXN3, are among the largest known human genes. Three of the genes (NRXN1, NRXN2, NRXN3) utilize two alternate promoters and include numerous alternatively spliced exons to generate thousands of distinct mRNA transcripts and protein isoforms. The majority of transcripts are produced from the upstream promoter and encode alpha-neurexin isoforms; a much smaller number of transcripts are produced from the downstream promoter and encode beta-neurexin isoforms. The alpha-neurexins contain EGF-like sequences and laminin G domains, and have been shown to interact with neurexophilins. The beta-neurexins lack EGF-like sequences and contain fewer laminin G domains than alpha-neurexins. NRXN3 have been linked to genetic predisposition towards a number of conditions such as alcohol or drug addiction, or obesity.

Description: A DNA sequence encoding the human NRXN3 beta isoform 2 (NP_620426.2) extracellular domain (Met 1-Thr 357) was expressed, with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 41350305
• NCBI GeneID: 9369
• NCBI Accession #: NP_620426.2
• NCBI GenBank Nucleotide #: NM_138970.4
• UniProt Accession #: Q9HDB5; Q6NUR0; Q8IUD8; A5PKW8; A8MPU5; B3KPM7; Q9Y4C0
• Molecular Weight: Mol_Mass: 36 kDa; AP_Mol_Mass: 50-60 kDa
• NCBI Official Full Name: neurexin 3 isoform 2
• NCBI Official Synonym Full Names: neurexin 3
• NCBI Official Symbol: NRXN3
• NCBI Official Synonym Symbols: C14orf60
• NCBI Protein Information: neurexin 3
• UniProt Protein Name: Neurexin-3-beta
• Protein Family: Neurexin
• UniProt Gene Name: NRXN3
• UniProt Synonym Gene Names: KIAA0743; NRXN3-CTF

NCBI Description

This gene encodes a member of a family of proteins that function in the nervous system as receptors and cell adhesion molecules. Extensive alternative splicing and the use of alternative promoters results in multiple transcript variants and protein isoforms for this gene, but the full-length nature of many of these variants has not been determined. Transcripts that initiate from an upstream promoter encode alpha isoforms, which contain epidermal growth factor-like (EGF-like) sequences and laminin G domains. Transcripts initiating from the downstream promoter encode beta isoforms, which lack EGF-like sequences. Genetic variation at this locus has been associated with a range of behavioral phenotypes, including alcohol dependence and autism spectrum disorder. [provided by RefSeq, Dec 2012]

Uniprot Description

NRXN3B: Neuronal cell surface protein that may be involved in cell recognition and cell adhesion. May play a role in angiogenesis. {ECO:0000250}. Belongs to the neurexin family. {ECO:0000305}. The cytoplasmic C-terminal region binds to CASK. Binds to neuroligins NLGN1, NLGN2 and NLGN3. Weakly interacts with CBLN1 and CBLN2. Very weak binding, if any, with CBLN4. {ECO:0000250}. 7 isoforms of the human protein are produced by alternative promoter

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q24.3-q31.1

Cellular Component: integral to plasma membrane

Molecular Function: cell adhesion molecule binding; receptor activity

Biological Process: adult behavior; axon guidance; learning; neuron adhesion; social behavior