STAT5B elisa kit

STAT5B (Phospho-Ser731) TFact DNA-Binding ELISA Kit

Cat. Num. AAA9502261

• Gene Names: STAT5B; STAT5
• Reactivity: Human, Mouse, Rat
• Synonyms: STAT5B; STAT5B (Phospho-Ser731) TFact DNA-Binding ELISA Kit; Signal transducer and activator of transcription 5B; STAT5B elisa kit

• Reactivity: Human, Mouse, Rat
• Specificity: The TFact STAT5B (Phospho-Ser731) DNA-Binding ELISA Kit detects endogenous levels of STAT5B only when phosphorylated at Ser731.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 787

• Samples: Cell lysate
• Assay Type: DNA-Binding ELISA
• Sensitivity: 10-30 ug of total protein/well
• Detection Method: Colorimetric 450 nm
• Subtype: Phospho
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The TFact STAT5B (Phospho-Ser731) DNA-Binding ELISA detects active STAT5B (Phospho-Ser731) in Hela and HepG2 Nuclear Extract. The Hela and HepG2 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by PMA (200nM) before harvest.)

NCBI and Uniprot Product Information

• NCBI GI #: 21618344
• NCBI GeneID: 6777
• NCBI Accession #: NP_036580.2
• NCBI GenBank Nucleotide #: NM_012448.3
• UniProt Accession #: P51692; Q8WWS8
• Molecular Weight: 89,866 Da
• NCBI Official Full Name: signal transducer and activator of transcription 5B
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5B
• NCBI Official Symbol: STAT5B
• NCBI Official Synonym Symbols: STAT5
• NCBI Protein Information: signal transducer and activator of transcription 5B
• UniProt Protein Name: Signal transducer and activator of transcription 5B
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT5B

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq, Jul 2008]

Uniprot Description

STAT5B: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases triggered by cytokines including IL2, IL3, GM-CSF, and various growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription.

Protein type: DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: chromatin binding; DNA binding transcription factor activity; glucocorticoid receptor binding; protein binding; protein dimerization activity; protein tyrosine kinase activity; signal transducer activity

Biological Process: 2-oxoglutarate metabolic process; allantoin metabolic process; cellular response to epidermal growth factor stimulus; cellular response to growth factor stimulus; cellular response to hormone stimulus; citrate metabolic process; creatine metabolic process; creatinine metabolic process; development of secondary female sexual characteristics; development of secondary male sexual characteristics; fatty acid metabolic process; female pregnancy; interleukin-7-mediated signaling pathway; isoleucine metabolic process; JAK-STAT cascade; lactation; lipid storage; luteinization; natural killer cell differentiation; negative regulation of apoptosis; negative regulation of erythrocyte differentiation; oxaloacetate metabolic process; peptidyl-tyrosine phosphorylation; Peyer's patch development; positive regulation of activated T cell proliferation; positive regulation of B cell differentiation; positive regulation of erythrocyte differentiation; positive regulation of gamma-delta T cell differentiation; positive regulation of inflammatory response; positive regulation of interleukin-2 biosynthetic process; positive regulation of mitotic cell cycle; positive regulation of multicellular organism growth; positive regulation of natural killer cell differentiation; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of natural killer cell proliferation; positive regulation of transcription from RNA polymerase II promoter; progesterone metabolic process; regulation of epithelial cell differentiation; regulation of multicellular organism growth; regulation of steroid metabolic process; regulation of transcription from RNA polymerase II promoter; response to estradiol; succinate metabolic process; T cell differentiation in thymus; T cell homeostasis; taurine metabolic process; transcription from RNA polymerase II promoter; valine metabolic process

Disease: Growth Hormone Insensitivity With Immunodeficiency