Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using YWHAB antibody.)
Rabbit YWHAB Polyclonal Antibody | anti-YWHAB antibody
YWHAB Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antigen affinity purification
Synonyms
YWHAB; Polyclonal Antibody; YWHAB Antibody; GW128; HS1; KCIP-1; 14-3-3; ; anti-YWHAB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Concentration
1mg/mL (varies by lot)
Sequence Length
63
Applicable Applications for anti-YWHAB antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Immunogen
Recombinant protein of human YWHAB
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-17288 / sc-13959 / sc-629 / sc-628
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using YWHAB antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using YWHAB antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney using YWHAB antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney using YWHAB antibody at dilution of 1:200 (400x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cell using YWHAB antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cell using YWHAB antibody.)
Related Product Information for anti-YWHAB antibody
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1, 2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau, and eta that have been identified in mammals. The initially described alpha and delta isoforms are confirmed to be phosphorylated forms of beta and zeta, respectively (3). Through their amino-terminal alpha helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3, 4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions, has been observed (4). 14-3-3 binding masks specific sequences of the target protein and therefore modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes that modify target protein function (4, 5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2, 3).
NCBI and Uniprot Product Information
Similar Products
Product Notes
The YWHAB (Catalog #AAA2517765) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The YWHAB Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's YWHAB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the YWHAB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YWHAB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
