Typical Testing Data/Standard Curve (for reference only)
Human VCAM-1 ELISA Kit | VCAM-1 elisa kit
VCAM-1 (human) ELISA Kit
Detection Range: 156 pg/ml - 10,000 pg/ml. Sensitivity: < 10 pg/ml.
This ELISA kit shows no cross-reactivity with other relevant proteins.
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Background: MyBioSource's human VCAM-1 ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for human VCAM-1 coated on a 96-well plate. Standards and test samples are added to the wells and VCAM-1 present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for VCAM-1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human VCAM-1 captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml - 10,000 pg/ml. Sensitivity: < 10 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Species reactivity-human
Application- Quantitative protein detection, establishing normal range, validation of antibody array results
NCBI and Uniprot Product Information
NCBI Description
binds integrin very late antigen (VLA4); plays a role in cell adhesion [RGD, Feb 2006]
Uniprot Description
VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral
Cellular Component: Golgi apparatus; extracellular space; microvillus; cell surface; endoplasmic reticulum; apical part of cell; early endosome; integral to membrane; podosome; sarcolemma; filopodium; external side of plasma membrane
Molecular Function: amine oxidase activity; integrin binding; cell adhesion molecule binding
Biological Process: response to nicotine; chronic inflammatory response; viral reproduction; heart development; cell-matrix adhesion; response to lipopolysaccharide; heterophilic cell adhesion; leukocyte adhesion; response to ethanol; response to zinc ion; membrane to membrane docking; response to hypoxia; positive regulation of T cell proliferation; response to ionizing radiation; amine metabolic process; leukocyte tethering or rolling; cell adhesion; response to nutrient; acute inflammatory response; aging
Research Articles on VCAM-1
Similar Products
Product Notes
The Human VCAM-1 vcam1 (Catalog #AAA843349) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA843349 ELISA Kit recognizes Human VCAM-1. Quantitative protein detection, establishing normal range. Researchers should empirically determine the suitability of the VCAM-1 vcam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VCAM-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
