Typical Testing Data/Standard Curve (for reference only)
Mouse Cathepsin B ELISA Kit | CTSB elisa kit
Cathepsin B (mouse) ELISA Kit
Reactivity
Mouse
Synonyms
Cathepsin B; Cathepsin B (mouse) ELISA Kit; CTSB (mouse) ELISA Kit; CTSB elisa kit
Reactivity
Mouse
Sequence Length
335
Application Notes
Quantitative protein detection, establishing normal range.
Samples
Serum & plasma (heparin, EDTA), Cell Culture Supernatants
Assay Type
Sandwich
Detection Method
Absorbance (450 nm)
Features & Benefits
Easy, convenient and time-saving method to assay for mouse Cathepsin B.
Detection Range: 156-10,000 pg/ml. Sensitivity: < 5 pg/ml.
This ELISA kit shows no cross-reactivity with other relevant proteins.
Detection Range: 156-10,000 pg/ml. Sensitivity: < 5 pg/ml.
This ELISA kit shows no cross-reactivity with other relevant proteins.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CTSB elisa kit
Product Information: Cathepsin B (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse Cathepsin B in serum, plasma & cell culture supernatants. Detection Range: 156 pg/ml - 10,000 pg/ml. 100 assays.
Background: MyBioSource's mouse Cathepsin B ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody form rat specific for Cathepsin B coated on a 96-well plate. Standards (NSO, H18-F339) and test samples are added to the wells and Cathepsin B present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for Cathepsin B is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse Cathepsin B captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml - 10,000 pg/ml. Sensitivity: < 5 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Species reactivity-mouse
Application- Quantitative protein detection, establishing normal range etc.
Background: MyBioSource's mouse Cathepsin B ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody form rat specific for Cathepsin B coated on a 96-well plate. Standards (NSO, H18-F339) and test samples are added to the wells and Cathepsin B present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for Cathepsin B is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse Cathepsin B captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml - 10,000 pg/ml. Sensitivity: < 5 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Species reactivity-mouse
Application- Quantitative protein detection, establishing normal range etc.
Product Categories/Family for CTSB elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,661 Da
NCBI Official Full Name
cathepsin B
NCBI Official Symbol
CTSB
NCBI Protein Information
cathepsin B
UniProt Protein Name
Cathepsin B
Protein Family
UniProt Gene Name
CTSB
UniProt Entry Name
CATB_BOVIN
Uniprot Description
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
Research Articles on CTSB
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Product Notes
The Mouse CTSB ctsb (Catalog #AAA843360) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA843360 ELISA Kit recognizes Mouse CTSB. Quantitative protein detection, establishing normal range. Researchers should empirically determine the suitability of the CTSB ctsb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cathepsin B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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