Typical Testing Data/Standard Curve (for reference only)
Rat Vascular Cell Adhesion Molecule 1 ELISA Kit | VCAM-1 elisa kit
Rat Vascular Cell Adhesion Molecule 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: VCAM-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VCAM-1 antibody and an VCAM-1-HRP conjugate. The assay sample and buffer are incubated together with VCAM-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VCAM-1 concentration since VCAM-1 from samples and VCAM-1-HRP conjugate compete for the anti-VCAM-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by VCAM-1 from the sample, fewer sites are left to bind VCAM-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VCAM-1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
binds integrin very late antigen (VLA4); plays a role in cell adhesion [RGD, Feb 2006]
Uniprot Description
VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral
Cellular Component: Golgi apparatus; extracellular space; microvillus; cell surface; endoplasmic reticulum; apical part of cell; early endosome; integral to membrane; podosome; sarcolemma; filopodium; external side of plasma membrane
Molecular Function: integrin binding; amine oxidase activity; cell adhesion molecule binding
Biological Process: response to nicotine; chronic inflammatory response; viral reproduction; cell-matrix adhesion; heart development; response to lipopolysaccharide; heterophilic cell adhesion; leukocyte adhesion; response to ethanol; response to zinc ion; membrane to membrane docking; response to hypoxia; positive regulation of T cell proliferation; amine metabolic process; response to ionizing radiation; cell adhesion; leukocyte tethering or rolling; response to nutrient; acute inflammatory response; aging
Research Articles on VCAM-1
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Product Notes
The Rat VCAM-1 vcam1 (Catalog #AAA726819) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA726819 ELISA Kit recognizes Rat VCAM-1. It is sometimes possible for the material contained within the vial of "Vascular Cell Adhesion Molecule 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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