Western Blot (WB)
SHISA9 positive control
SHISA9 Antibody Positive Control
Reactivity
Mouse
Applications
Western Blot
Synonyms
SHISA9; SHISA9 Antibody Positive Control; CKAMP44; shisa family member 9 antibody; SHISA9 positive control
Reactivity
Mouse
Form/Format
Western Blot Positive Control in Ready-to-Use Buffer
Sequence Length
423
Applicable Applications for SHISA9 positive control
Western Blot (WB)
Application Notes
ELISA: 1:10,000
Dot Blot: 1:10,000
Western Blot: 1:500
Dot Blot: 1:10,000
Western Blot: 1:500
Preparation and Storage
Store at -20°C for long term storage.
Western Blot (WB)
Western Blot (WB)
Related Product Information for SHISA9 positive control
Description: CKAMP44/SHISA9 in SDS-PAGE sample buffer for ready-to-use application in SDS-PAGE
Relevance: Cytosine-knot AMPAR modulating protein 44 (CKAMP44A) is a novel protein that was recently identified as a brain-specific type I transmembrane protein that associates with AMPA receptors in synaptic spines (1). AMPA receptors (AMPARs) mediate fast synaptic transmissions in the vertebrate central nervous system (2). AMPARs are composed of four types of subunits, named as GluR1, GluR2, Glur3, and GluR4, which combine to form tetramers (3, 4). Several AMPAR-interacting proteins have been identified that affect the receptors' subcellular localization, synaptic stabilization, and kinetics, these include transmembrane AMPAR regulatory proteins (TARPs), Sol-1 and cornichons (5-7). CKAMP44A, located on human and mouse chromosome 16, was recently identified as another AMPAR-interacting protein (l). CKAMP44A was shown to modulate short-term plasticity at some excitatory synapses. ln Xenopus Oocytes, expression of CKAMP44A reduced GluR1 and GluR2 mediated steady-state currents, but it did not affect kainite or NMDA receptor-mediated currents (1).
CKAMP44A contains an extracellular N-terminal cysteine-rich motif with eight cysteines that are highly conserved across vertebrate species. Six of these eight cysteine residues might function as stabilizers for the Cys-knot structure of the CKAMP44A (l). RT-PCR analysis from various mouse tissues RNA revealed two splice variants, CKAMP44Aa and CKAMP44Ab, which differ by only 48 bases (1). This CKAMP44A-specific antibody recognizes CKAMP44Aa. Its expression is restricted to the brain; its mRNA is present in most brain structures during embryonic and postnatal development. CKAMP44Aa is approximately a 52kDa protein (432 amino acids).
Relevance: Cytosine-knot AMPAR modulating protein 44 (CKAMP44A) is a novel protein that was recently identified as a brain-specific type I transmembrane protein that associates with AMPA receptors in synaptic spines (1). AMPA receptors (AMPARs) mediate fast synaptic transmissions in the vertebrate central nervous system (2). AMPARs are composed of four types of subunits, named as GluR1, GluR2, Glur3, and GluR4, which combine to form tetramers (3, 4). Several AMPAR-interacting proteins have been identified that affect the receptors' subcellular localization, synaptic stabilization, and kinetics, these include transmembrane AMPAR regulatory proteins (TARPs), Sol-1 and cornichons (5-7). CKAMP44A, located on human and mouse chromosome 16, was recently identified as another AMPAR-interacting protein (l). CKAMP44A was shown to modulate short-term plasticity at some excitatory synapses. ln Xenopus Oocytes, expression of CKAMP44A reduced GluR1 and GluR2 mediated steady-state currents, but it did not affect kainite or NMDA receptor-mediated currents (1).
CKAMP44A contains an extracellular N-terminal cysteine-rich motif with eight cysteines that are highly conserved across vertebrate species. Six of these eight cysteine residues might function as stabilizers for the Cys-knot structure of the CKAMP44A (l). RT-PCR analysis from various mouse tissues RNA revealed two splice variants, CKAMP44Aa and CKAMP44Ab, which differ by only 48 bases (1). This CKAMP44A-specific antibody recognizes CKAMP44Aa. Its expression is restricted to the brain; its mRNA is present in most brain structures during embryonic and postnatal development. CKAMP44Aa is approximately a 52kDa protein (432 amino acids).
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
cystine-knot AMPAR modulating protein 44a
UniProt Protein Name
Protein shisa-9
Protein Family
UniProt Gene Name
Shisa9
UniProt Synonym Gene Names
CKAMP44
UniProt Entry Name
SHSA9_MOUSE
Uniprot Description
SHISA9: Regulator of short-term neuronal synaptic plasticity in the dentate gyrus. Associates with AMPA receptors (ionotropic glutamate receptors) in synaptic spines and promotes AMPA receptor desensitization at excitatory synapses. Belongs to the shisa family. SHISA9 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: cell junction; cell projection; integral to membrane; ionotropic glutamate receptor complex; membrane; plasma membrane; synapse
Molecular Function: PDZ domain binding
Biological Process: regulation of short-term neuronal synaptic plasticity
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Product Notes
The SHISA9 shisa9 (Catalog #AAA542637) is a Positive Control and is intended for research purposes only. The product is available for immediate purchase. The SHISA9 Antibody Positive Control reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SHISA9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). ELISA: 1:10,000 Dot Blot: 1:10,000 Western Blot: 1:500. Researchers should empirically determine the suitability of the SHISA9 shisa9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHISA9, Positive Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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