Typical Testing Data/Standard Curve (for reference only)
Rat Vascular cell adhesion molecule 1, VCAM-1 ELISA Kit | VCAM-1 elisa kit
Rat Vascular cell adhesion molecule 1, VCAM-1 ELISA Kit
Gene Names
Vcam1; VCAM1B
Reactivity
Rat
Synonyms
Vascular cell adhesion molecule 1; VCAM-1; Rat Vascular cell adhesion molecule 1; VCAM-1 ELISA Kit; VCAM-1 elisa kit
Reactivity
Rat
Sequence Length
739
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05ng/ml - 20ng/ml
Sensitivity
0.032ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for VCAM-1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Vascuolar Cell Adhesion Molecule 1 (also known as VCAM-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat VCAM-1 antibody. VCAM-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat VCAM-1 Antibody is added and binds to VCAM-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VCAM-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat VCAM-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat VCAM-1 antibody. VCAM-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat VCAM-1 Antibody is added and binds to VCAM-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VCAM-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat VCAM-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
81,246 Da
NCBI Official Full Name
vascular cell adhesion protein 1
NCBI Official Synonym Full Names
vascular cell adhesion molecule 1
NCBI Official Symbol
Vcam1
NCBI Official Synonym Symbols
VCAM1B
NCBI Protein Information
vascular cell adhesion protein 1
UniProt Protein Name
Vascular cell adhesion protein 1
UniProt Gene Name
Vcam1
UniProt Synonym Gene Names
Vcam-1; V-CAM 1; VCAM-1
NCBI Description
binds integrin very late antigen (VLA4); plays a role in cell adhesion [RGD, Feb 2006]
Uniprot Description
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Research Articles on VCAM-1
Similar Products
Product Notes
The Rat VCAM-1 vcam1 (Catalog #AAA164506) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA164506 ELISA Kit recognizes Rat VCAM-1. It is sometimes possible for the material contained within the vial of "Vascular cell adhesion molecule 1, VCAM-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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