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Typical Testing Data/Standard Curve (for reference only)

Human Serpin G1 ELISA Kit

Human Serpin G1 PicoKine ELISA Kit

Reactivity
Human
Synonyms
Serpin G1; Human Serpin G1 PicoKine ELISA Kit; Human Serpin G1; Serpin G1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Natural and recombinant Human SERPINF2
Sequence Length
154
Samples
Cell Culture Supernates, Serum And Plasma (Heparin, Edta, Citrate)
Detection Range
1.56ng/ml-100ng/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Serpin G1 elisa kit
Principle of the Assay: The Picokine Human SERPINF2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human SERPINF2 with a 96-well strip plate that is pre-coated with antibody specific for SERPINF2. The detection antibody is a biotinylated antibody specific for SERPINF2. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human SERPINF2 with immunogen: Expression system for standard: NSO, Immunogen sequence: N40-K491. The kit is analytically validated with ready to use reagents. To measure Human SERPINF2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human SERPINF2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human SERPINF2 in the sample!!Background/Introduction: Human SerpinF2 encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
NCBI Official Full Name
serpin peptidase inhibitor, clade G (C1 inhibitor), member 1, partial

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Product Notes

The Human Serpin G1 (Catalog #AAA1751584) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751584 ELISA Kit recognizes Human Serpin G1. It is sometimes possible for the material contained within the vial of "Serpin G1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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