Serpin A12 ELISA Kit | SERPINA12 elisa kit

Human Serpin A12 PicoKine ELISA Kit

Cat. Num. AAA1751661

• Gene Names: SERPINA12; OL-64
• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Synonyms: Serpin A12; Human Serpin A12 PicoKine ELISA Kit; Human Serpin A12; OL-64; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin; SERPINA12; Serpin family A member 12; SERPINA12 elisa kit

• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Human Serpin A1
• Sequence Length: 414

• Samples: Cell Culture Supernates, Serum, Plasma (Heparin, Edta) And Urine
• Detection Range: 1.56ng/ml-100ng/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months.; Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SERPINA12 elisa kit

Principle of the Assay: The Picokine Human Serpin A1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human Serpin A1 with a 96-well strip plate that is pre-coated with antibody specific for Serpin A1. The detection antibody is a biotinylated antibody specific for Serpin A1. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human Serpin A1 with immunogen: Expression system for standard: NSO,E25-K418. The kit is analytically validated with ready to use reagents. To measure Human Serpin A1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human Serpin A1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human Serpin A1 in the sample!!Background/Introduction: Alpha-1-antitrypsin or alpha1-antitrypsin (A1AT, A1A, or AAT) is a protein belonging to the serpin superfamily. It is encoded in humans by the SERPINA1 gene. The gene is located on the long arm of the fourteenth chromosome (14q32.1).The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Mutations in these areas can lead to non-functional proteins that can polymerise and accumulate in the liver(infantile hepatic cirrhosis).

NCBI and Uniprot Product Information

• NCBI GI #: 751130493
• NCBI GeneID: 145264
• NCBI Accession #: NP_001291390.1
• NCBI GenBank Nucleotide #: NM_001304461.1
• UniProt Accession #: Q8IW75
• Molecular Weight: 47,175 Da
• NCBI Official Full Name: serpin A12
• NCBI Official Synonym Full Names: serpin family A member 12
• NCBI Official Symbol: SERPINA12
• NCBI Official Synonym Symbols: OL-64
• NCBI Protein Information: serpin A12
• UniProt Protein Name: Serpin A12
• Protein Family: Serpin
• UniProt Gene Name: SERPINA12
• UniProt Synonym Gene Names: Vaspin

Uniprot Description

Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.