Human ckmm ELISA Kit | CKMM elisa kit

Human ckmm PicoKine ELISA Kit

Cat. Num. AAA1751625

• Reactivity: Human
• Synonyms: ckmm; Human ckmm PicoKine ELISA Kit; Human ckmm; CKMM elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CILP

• Samples: Cell Culture Supernates, Serum And Plasma(Heparin, Edta, Citrate)
• Detection Range: 93.8pg/ml-6000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CKMM elisa kit

Principle of the Assay: The Picokine Human CILP Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CILP with a 96-well strip plate that is pre-coated with antibody specific for CILP. The detection antibody is a biotinylated antibody specific for CILP. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human CILP with immunogen: Expression system for standard: NSO, Immunogen sequence: M1-R720. The kit is analytically validated with ready to use reagents. To measure Human CILP, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human CILP in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CILP in the sample!!Background/Introduction: Cartilage intermediate layer protein 1 is a protein that in humans is encoded by the CILP gene. It is mapped to 15q22.31. Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease.