Human Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) ELISA Kit | RPS6Kb1 elisa kit

Human Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) ELISA Kit

Cat. Num. AAA2020312

• Gene Names: Rps6kb1; S6K1; 70kDa; p70s6k; AA959758; AI256796; AI314060; p70/85s6k; 2610318I15Rik; 4732464A07Rik
• Reactivity: Human
• Synonyms: Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1); Human Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) ELISA Kit; PS6K; S6K; S6K1; STK14A; p70(S6K)-alpha; p70-S6K; p70-alpha; P70-S6 Kinase 1; 70 kDa ribosomal protein S6 kinase 1; Serine/threonine-protein kinase 14A; RPS6Kb1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1).; No significant cross-reactivity or interference between Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) and analogues was observed.
• Sequence Length: 525

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RPS6Kb1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RPS6Kb1 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 166999987
• NCBI GeneID: 72508
• NCBI Accession #: NP_001107806.1
• NCBI GenBank Nucleotide #: NM_001114334.1
• UniProt Accession #: Q8BSK8; Q5SWG1; Q8CHX0
• Molecular Weight: 56,157 Da
• NCBI Official Full Name: ribosomal protein S6 kinase beta-1 isoform 1
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, polypeptide 1
• NCBI Official Symbol: Rps6kb1
• NCBI Official Synonym Symbols: S6K1; 70kDa; p70s6k; AA959758; AI256796; AI314060; p70/85s6k; 2610318I15Rik; 4732464A07Rik
• NCBI Protein Information: ribosomal protein S6 kinase beta-1
• UniProt Protein Name: Ribosomal protein S6 kinase beta-1
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: Rps6kb1
• UniProt Synonym Gene Names: S6K-beta-1; S6K1; P70S6K1; p70-S6K 1; S6K; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA
• UniProt Entry Name: KS6B1_MOUSE

Uniprot Description

p70S6K: an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported.

Protein type: Kinase, protein; EC 2.7.11.1; Translation; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; RSK family; p70 subfamily

Cellular Component: nucleoplasm; mitochondrial outer membrane; neuron projection; cell surface; membrane; mitochondrion; perinuclear region of cytoplasm; cytoplasm; synapse; intracellular; cell junction; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; protein serine/threonine/tyrosine kinase activity; kinase activity; ribosomal protein S6 kinase activity; peptide binding; ATP binding; protein kinase activity

Biological Process: behavioral fear response; TOR signaling pathway; positive regulation of translation; apoptosis; positive regulation of smooth muscle cell proliferation; negative regulation of insulin receptor signaling pathway; signal transduction; cell cycle; positive regulation of mitotic cell cycle; positive regulation of smooth muscle cell migration; protein amino acid phosphorylation; response to insulin stimulus; germ cell development; positive regulation of translational initiation; regulation of translation; cellular response to insulin stimulus; protein kinase B signaling cascade; regulation of glucose import; positive regulation of skeletal muscle growth; phosphorylation; G1/S transition of mitotic cell cycle; negative regulation of apoptosis