Typical Testing Data/Standard Curve (for reference only)
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Aflibercept ELISA Kit
Aflibercept ELISA Kit
Synonyms
Aflibercept; Aflibercept ELISA Kit; Eylea; Zaltrap; Aflibercept elisa kit
Specificity
There is no cross reaction with any other proteins present in native human serum. A screening test was performed with 48 different native human sera. All produced OD450/620 nm values less than the mean OD of standard D. No cross reaction was observed with the other therapeutic antibodies tested (Tocilizumab, Cetuximab, Etanercept, Infliximab, Rituximab and Trastuzumab) at concentrations up to 2 mg/mL. However, Bevacizumab resulted in full cross-reaction as expected. Ranibizumab, another anti-VEGF therapeutic antibody consisted of Fab fragment only, produced a mean OD less than the mean OD of standard D.
Samples
Serum and plasma
Assay Type
Sandwich
Intra-assay Precision
<10%
Inter-assay Precision
<10%
Preparation and Storage
The kit is shipped at ambient temperature and should be stored at 2-8 degree C. Keep away from heat or direct sun light. The storage and stability of specimen and prepared reagents is stated in the corresponding chapters. The microtiter strips are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2–8 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for Aflibercept elisa kit
Intended Uses: Enzyme immunoassay for the determination of free Aflibercept in serum and plasma.
Principle of the Assay: This ELISA assay is based on sandwich type ELISA. Diluted standards and samples (serum or plasma) are incubated in the microtiter plate coated with recombinant human vascular endothelial growth factor-A (rhVEGF-A). After incubation, the wells are washed. A horseradish peroxidase (HRP)conjugated anti-human IgG monoclonal antibody is added and binds to the Fc part of Aflibercept pre-captured by the rhVEGF-A on the surface of the wells. Following incubation, the wells are washed, and the bound enzymatic activity is detected by addition of chromogen-substrate. The color developed is proportional to the amount of Aflibercept in the sample or standard. Results of samples can be determined by using the standard curve.
Principle of the Assay: This ELISA assay is based on sandwich type ELISA. Diluted standards and samples (serum or plasma) are incubated in the microtiter plate coated with recombinant human vascular endothelial growth factor-A (rhVEGF-A). After incubation, the wells are washed. A horseradish peroxidase (HRP)conjugated anti-human IgG monoclonal antibody is added and binds to the Fc part of Aflibercept pre-captured by the rhVEGF-A on the surface of the wells. Following incubation, the wells are washed, and the bound enzymatic activity is detected by addition of chromogen-substrate. The color developed is proportional to the amount of Aflibercept in the sample or standard. Results of samples can be determined by using the standard curve.
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Product Notes
The Aflibercept (Catalog #AAA495554) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Aflibercept, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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