Typical Testing Data/Standard Curve (for reference only)
Human RPS6Kbeta1 (Ribosomal Protein S6 Kinase Beta 1) CLIA Kit | RPS6Kb1 clia kit
Human RPS6Kbeta1 (Ribosomal Protein S6 Kinase Beta 1) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to RPS6Kbeta1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RPS6Kbeta1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain RPS6Kbeta1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of RPS6Kbeta1. You can calculate the concentration of RPS6Kbeta1 in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
p70S6K: an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; Translation; EC 2.7.11.1; AGC group; RSK family; p70 subfamily
Cellular Component: nucleoplasm; mitochondrial outer membrane; neuron projection; cell surface; membrane; mitochondrion; perinuclear region of cytoplasm; cytoplasm; synapse; nucleus; cell junction; cytosol
Molecular Function: identical protein binding; protein phosphatase 2A binding; nucleotide binding; peptide binding; protein kinase activity; PDZ domain binding; transferase activity; protein serine/threonine kinase activity; protein binding; protein serine/threonine/tyrosine kinase activity; kinase activity; ribosomal protein S6 kinase activity; ATP binding
Biological Process: TOR signaling pathway; behavioral fear response; positive regulation of translation; apoptosis; positive regulation of smooth muscle cell proliferation; negative regulation of insulin receptor signaling pathway; positive regulation of mitotic cell cycle; signal transduction; cell cycle; positive regulation of smooth muscle cell migration; protein amino acid phosphorylation; germ cell development; response to insulin stimulus; regulation of translation; positive regulation of translational initiation; cellular response to insulin stimulus; protein kinase B signaling cascade; regulation of glucose import; positive regulation of skeletal muscle growth; phosphorylation; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
Research Articles on RPS6Kb1
Similar Products
Product Notes
The Human RPS6Kb1 rps6kb1 (Catalog #AAA2532212) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2532212 CLIA Kit recognizes Human RPS6Kb1. It is sometimes possible for the material contained within the vial of "RPS6Kbeta1 (Ribosomal Protein S6 Kinase Beta 1), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
