Human Ribosomal Protein S6 Kinase Beta 1 ELISA Kit | RPS6Kbeta1 elisa kit

Human Ribosomal Protein S6 Kinase Beta 1 ELISA Kit

Cat. Num. AAA763572

• Gene Names: Rps6kb1; S6K1; 70kDa; p70s6k; AA959758; AI256796; AI314060; p70/85s6k; 2610318I15Rik; 4732464A07Rik
• Reactivity: Human
• Synonyms: Ribosomal Protein S6 Kinase Beta 1; Human Ribosomal Protein S6 Kinase Beta 1 ELISA Kit; RPS6Kb1 ; RPS6Kbeta1 elisa kit

• Reactivity: Human
• Sequence Length: 525

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for RPS6Kbeta1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human RPS6Kbeta1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RPS6Kbeta1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RPS6Kbeta1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human RPS6Kbeta1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 166999987
• NCBI GeneID: 72508
• NCBI Accession #: NP_001107806.1
• NCBI GenBank Nucleotide #: NM_001114334.1
• Molecular Weight: 56,157 Da
• NCBI Official Full Name: ribosomal protein S6 kinase beta-1 isoform 1
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, polypeptide 1
• NCBI Official Symbol: Rps6kb1
• NCBI Official Synonym Symbols: S6K1; 70kDa; p70s6k; AA959758; AI256796; AI314060; p70/85s6k; 2610318I15Rik; 4732464A07Rik
• NCBI Protein Information: ribosomal protein S6 kinase beta-1
• UniProt Protein Name: Ribosomal protein S6 kinase beta-1
• UniProt Gene Name: Rps6kb1
• UniProt Synonym Gene Names: S6K-beta-1; S6K1; P70S6K1; p70-S6K 1; S6K; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA
• UniProt Entry Name: KS6B1_MOUSE

Uniprot Description

p70S6K: an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported.

Protein type: Protein kinase, AGC; Translation; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; AGC group; RSK family; p70 subfamily

Cellular Component: cell junction; cell surface; cytoplasm; cytosol; membrane; mitochondrial outer membrane; mitochondrion; neuron projection; nucleoplasm; nucleus; perinuclear region of cytoplasm; synapse

Molecular Function: ATP binding; identical protein binding; kinase activity; nucleotide binding; PDZ domain binding; peptide binding; protein binding; protein kinase activity; protein phosphatase 2A binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; ribosomal protein S6 kinase activity; transferase activity

Biological Process: apoptosis; behavioral fear response; cell cycle; cellular response to insulin stimulus; G1/S transition of mitotic cell cycle; germ cell development; negative regulation of apoptosis; negative regulation of insulin receptor signaling pathway; phosphorylation; positive regulation of mitotic cell cycle; positive regulation of skeletal muscle growth; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of translation; positive regulation of translational initiation; protein amino acid phosphorylation; protein kinase B signaling cascade; regulation of glucose import; regulation of translation; response to insulin stimulus; signal transduction; TOR signaling pathway