Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ras-related protein Rab-5B (RAB5B) Standard Curve.)
Human Ras-Related Protein Rab-5B (RAB5B) ELISA Kit | RAB5B elisa kit
Human Ras-Related Protein Rab-5B (RAB5B) ELISA Kit
Reactivity
Human
Synonyms
Ras-Related Protein Rab-5B (RAB5B); Human Ras-Related Protein Rab-5B (RAB5B) ELISA Kit; RAB5B elisa kit
Reactivity
Human
Specificity
Human Ras-related protein Rab-5B (RAB5B) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAB5B. No significant cross-reactivity or interference between Human RAB5B and analogues was observed.
Sequence Length
215
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ras-related protein Rab-5B (RAB5B) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ras-related protein Rab-5B (RAB5B) Standard Curve.)
Related Product Information for RAB5B elisa kit
Description: This Human Ras-related protein Rab-5B (RAB5B) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB5B in samples. An antibody specific for RAB5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAB5B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB5B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB5B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: A subset of these GTPases plays a central role in membrane trafficking. Each of the several proteins of this subfamily that have been identified is thought to regulate vesicular trafficking at a specific subcellular compartment. The subcellular location of several RAB proteins has been determined by immunohistochemical methods. For example, RAB2 is found in the intermediate recycling pathway between the endoplasmic reticulum and the Golgi complex. RAB6 is distributed in the medial and trans Golgi. RAB4 and RAB5A are associated with the plasma membrane and early endosomes. The newly identified RAB protein was 81% identical to human RAB5, the canine counterpart of which had been localized to the plasma membrane and early endosomes. RAB5B is presumably involved in vesicular trafficking at the plasma membrane.
Background: A subset of these GTPases plays a central role in membrane trafficking. Each of the several proteins of this subfamily that have been identified is thought to regulate vesicular trafficking at a specific subcellular compartment. The subcellular location of several RAB proteins has been determined by immunohistochemical methods. For example, RAB2 is found in the intermediate recycling pathway between the endoplasmic reticulum and the Golgi complex. RAB6 is distributed in the medial and trans Golgi. RAB4 and RAB5A are associated with the plasma membrane and early endosomes. The newly identified RAB protein was 81% identical to human RAB5, the canine counterpart of which had been localized to the plasma membrane and early endosomes. RAB5B is presumably involved in vesicular trafficking at the plasma membrane.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Ras-related protein Rab-5B
NCBI Official Synonym Full Names
RAB5B, member RAS oncogene family
NCBI Official Symbol
RAB5B
NCBI Protein Information
ras-related protein Rab-5B
UniProt Protein Name
Ras-related protein Rab-5B
Protein Family
UniProt Gene Name
RAB5B
UniProt Entry Name
RAB5B_HUMAN
Uniprot Description
RAB5B: Protein transport. Probably involved in vesicular traffic. Belongs to the small GTPase superfamily. Rab family.
Protein type: G protein; G protein, monomeric, Rab; G protein, monomeric
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: membrane; endocytic vesicle; early endosome membrane; early endosome; plasma membrane; melanosome; intracellular; endosome
Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; GTP-dependent protein binding
Biological Process: antigen processing and presentation; intracellular protein transport; endosome organization and biogenesis; metabolic process; regulation of endocytosis; plasma membrane to endosome transport; Rab protein signal transduction
Research Articles on RAB5B
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Product Notes
The Human RAB5B rab5b (Catalog #AAA9714654) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714654 ELISA Kit recognizes Human RAB5B. It is sometimes possible for the material contained within the vial of "Ras-Related Protein Rab-5B (RAB5B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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