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Western Blot (WB) (Western blot analysis of extracts from Rat spleen lysate, using CACNG1 antibody.)

Rabbit CACNG1 Polyclonal Antibody | anti-CACNG1 antibody

CACNG1 Antibody

Gene Names
CACNG1; CACNLG
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CACNG1; Polyclonal Antibody; CACNG1 Antibody; CACNLG; CACNLG calcium channel neuronal dihydrpyridine sensitive gamma subunit; Calcium channel L type gamma polypeptide; Calcium channel voltage dependent gamma subunit 1; CCG1_HUMAN; Dihydropyridine sensitive L type skeletal muscle calcium channel gamma subunit; Dihydropyridine sensitive L type skeletal muscle calcium channel subunit gamma; Dihydropyridine-sensitive L-type; fb43f02; fi19g08; L type calcium channel gamma polypeptide; MGC117703; skeletal muscle calcium channel subunit gamma; Voltage dependent calcium channel gamma 1 subunit 2; Voltage-dependent calcium channel gamma-1 subunit; wu:fb43f02; zgc:55364; anti-CACNG1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CACNG1 antibody detects endogenous levels of total CACNG1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
222
Applicable Applications for anti-CACNG1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Membrane.
Tissue Specificity
Skeletal muscle.
Predicted Cross Reactivity
Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Bovine (82%), Horse (100%), Rabbit (100%), Dog (91%), Chicken (100%), Xenopus (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Rat spleen lysate, using CACNG1 antibody.)

Western Blot (WB) (Western blot analysis of extracts from Rat spleen lysate, using CACNG1 antibody.)

Immunofluorescene (IF)

(MBS9606066 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Immunofluorescene (IF) (MBS9606066 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Western Blot (WB)

(Western blot analysis of extracts from rat spleen, using CACNG1 Antibody.)

Western Blot (WB) (Western blot analysis of extracts from rat spleen, using CACNG1 Antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using CACNG1 antibody.)

Western Blot (WB) (Western blot analysis of extracts from HeLa cells, using CACNG1 antibody.)
Related Product Information for anti-CACNG1 antibody
Function: This protein is a subunit of the dihydropyridine (DHP) sensitive calcium channel. Plays a role in excitation-contraction coupling. The skeletal muscle DHP-sensitive Ca2+ channel may function only as a multiple subunit complex.
Subunit Structure: The L-type calcium channel is composed of five subunits: alpha-1, alpha-2/delta, beta and gamma.
Similarity: Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
786
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 25 kD
Predicted: 26 kDa
NCBI Official Full Name
voltage-dependent calcium channel gamma-1 subunit
NCBI Official Synonym Full Names
calcium voltage-gated channel auxiliary subunit gamma 1
NCBI Official Symbol
CACNG1
NCBI Official Synonym Symbols
CACNLG
NCBI Protein Information
voltage-dependent calcium channel gamma-1 subunit
UniProt Protein Name
Voltage-dependent calcium channel gamma-1 subunit
UniProt Gene Name
CACNG1

NCBI Description

Voltage-dependent calcium channels are composed of five subunits. The protein encoded by this gene represents one of these subunits, gamma, and is one of two known gamma subunit proteins. This particular gamma subunit is part of skeletal muscle 1,4-dihydropyridine-sensitive calcium channels and is an integral membrane protein that plays a role in excitation-contraction coupling. This gene is part of a functionally diverse eight-member protein subfamily of the PMP-22/EMP/MP20 family and is located in a cluster with two family members that function as transmembrane AMPA receptor regulatory proteins (TARPs). [provided by RefSeq, Dec 2010]

Uniprot Description

Regulatory subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents in skeletal muscle. Regulates channel inactivation kinetics.

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Product Notes

The CACNG1 cacng1 (Catalog #AAA9606066) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CACNG1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CACNG1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the CACNG1 cacng1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CACNG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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