Mouse anti-Human PP5 Monoclonal Antibody | anti-PP5 antibody

Mouse Anti-Human PP5 Monoclonal IgG2a

Cat. Num. AAA807301

• Gene Names: PPP5C; PP5; PPT; PPP5
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein G Purified
• Synonyms: PP5; Monoclonal Antibody; Mouse Anti-Human PP5 Monoclonal IgG2a; PPP5 Antibody; PPT Antibody; Protein phosphatase T Antibody; Serine/threonine protein phosphatase 5 Antibody; PP5 Antibody: ATTO 390; Mouse Anti-Human PP5 Monoclonal; Clone 2E11; PP5 Antibody; anti-PP5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 2E11
• Purity/Purification: Protein G Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 499

• Applicable Applications for anti-PP5 antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:1000; Optimal dilutions should be determined by the user.
• Immunogen: Full length human PP5 protein
• Immunogen Species: Human
• COA: 1 ug/ml was sufficient for detection of PP5 in 15 ug of human A431 lysates by ECL analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Conjugate: ATTO 390
• Cellular Localization: Nucleus, Cytoplasm
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western Blot analysis of Human A431, HEK293, and Jurkat cell lysates showing detection of ~58 kDa PP5 protein using Mouse Anti-PP5 Monoclonal Antibody, Clone 2E11. Lane 1: MW Ladder. Lane 2: Human A431 (15 ug). Lane 3: Human HEK293 (15 ug). Lane 4: Human Jurkat (15 ug). Load: 15 ug. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~58 kDa.)

Related Product Information for anti-PP5 antibody

Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues, potentially playing a role in the regulation of cell growth and differentiation. PP5 or PPT is predominantly a nuclear protein and interacts with CDC16 and CDC27. It is found in association with several proteins that influence intracellular signlaing-cascades intitiated by hormones (glucocorticoids) or cellular stress (hypoxia, oxidative stress and DNA Damage). It phosphorylates serine residues of skeletal muscle phosphorylase and histone H1. It may also be involved in mitosis and RNA biogenesis regulation (1,2).

Product Categories/Family for anti-PP5 antibody

Cell Signaling; Epigenetics

References

1. Butzow R., et al. (1988) J Lab Clin Med. 111(2): 249-256.

NCBI and Uniprot Product Information

• NCBI GI #: 5453958
• NCBI GeneID: 5536
• NCBI Accession #: NP_006238.1
• NCBI GenBank Nucleotide #: NM_006247.3
• UniProt Accession #: P53041; Q16722; Q53XV2
• Molecular Weight: Detects ~57kDa.
• NCBI Official Full Name: serine/threonine-protein phosphatase 5 isoform 1
• NCBI Official Synonym Full Names: protein phosphatase 5 catalytic subunit
• NCBI Official Symbol: PPP5C
• NCBI Official Synonym Symbols: PP5; PPT; PPP5
• NCBI Protein Information: serine/threonine-protein phosphatase 5
• UniProt Protein Name: Serine/threonine-protein phosphatase 5
• UniProt Gene Name: PPP5C
• UniProt Synonym Gene Names: PPP5; PP5; PP-T; PPT
• UniProt Entry Name: PPP5_HUMAN

NCBI Description

This gene encodes a serine/threonine phosphatase which is a member of the protein phosphatase catalytic subunit family. Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues; many of these pathways are involved in the regulation of cell growth and differentiation. The product of this gene has been shown to participate in signaling pathways in response to hormones or cellular stress, and elevated levels of this protein may be associated with breast cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]

Uniprot Description

PPP5C: a Ser/Thr phosphatase. Plays a role in the survival of cells in a low oxygen stress by suppressing a hypoxia-induced ASK-1/MKK4/JNK signaling cascade that promotes apoptosis. Has been reported to interact with DNA-PK, dephosphorylating it at two functional sites. May play a role in the regulation of RNA biogenesis and/or mitosis.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; nucleoplasm; nucleus; perikaryon; plasma membrane; protein complex

Molecular Function: ADP binding; ATP binding; G-protein alpha-subunit binding; heat shock protein binding; identical protein binding; lipid binding; metal ion binding; microtubule binding; phosphoprotein phosphatase activity; protein binding; protein serine/threonine phosphatase activity; RNA binding; signal transducer activity

Biological Process: activation of MAPKK activity; axon guidance; DNA repair; double-strand break repair; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; histone dephosphorylation; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; mitosis; negative regulation of protein amino acid phosphorylation; nerve growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein amino acid dephosphorylation; protein heterooligomerization; Ras protein signal transduction; response to lead ion; response to morphine; small GTPase mediated signal transduction; transcription, DNA-dependent; vascular endothelial growth factor receptor signaling pathway