Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ras-related protein Rab-5C (RAB5C) Standard Curve.)
Human Ras-Related Protein Rab-5C (RAB5C) ELISA Kit | RAB5C elisa kit
Human Ras-Related Protein Rab-5C (RAB5C) ELISA Kit
Gene Names
RAB5C; RABL; L1880; RAB5L; RAB5CL
Reactivity
Human
Synonyms
Ras-Related Protein Rab-5C (RAB5C); Human Ras-Related Protein Rab-5C (RAB5C) ELISA Kit; RAB5C; MGC117217; MGC138857; RAB5CL; RABL; RAB; member of RAS oncogene family-like; member of RAS oncogene family; RAB5C elisa kit
Reactivity
Human
Specificity
Human Ras-related protein Rab-5C (RAB5C) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAB5C. No significant cross-reactivity or interference between Human RAB5C and analogues was observed.
Sequence Length
249
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ras-related protein Rab-5C (RAB5C) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ras-related protein Rab-5C (RAB5C) Standard Curve.)
Related Product Information for RAB5C elisa kit
Description: This Human Ras-related protein Rab-5C (RAB5C) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB5C in samples. An antibody specific for RAB5C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAB5C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB5C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB5C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment. Han et al. (1996) isolated fetal lung cDNAs encoding a RAB protein that they designated RABL (RAB5-like). The predicted 216-amino acid RABL protein shares 86% identity with RAB5A and RAB5B and contains the conserved GTP-binding site characteristic of RAS proteins. RT-PCR analysis revealed that RABL is expressed ubiquitously. By analysis of cloned segments, Albertsen et al. (1994) determined that the RABL gene, which they called BC1-16, is located in the BRCA1 gene region on chromosome 17q12-q21. Han et al. (1996) refined the map position to 17q21.2 using FISH.
Background: Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment. Han et al. (1996) isolated fetal lung cDNAs encoding a RAB protein that they designated RABL (RAB5-like). The predicted 216-amino acid RABL protein shares 86% identity with RAB5A and RAB5B and contains the conserved GTP-binding site characteristic of RAS proteins. RT-PCR analysis revealed that RABL is expressed ubiquitously. By analysis of cloned segments, Albertsen et al. (1994) determined that the RABL gene, which they called BC1-16, is located in the BRCA1 gene region on chromosome 17q12-q21. Han et al. (1996) refined the map position to 17q21.2 using FISH.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ras-related protein Rab-5C isoform b
NCBI Official Synonym Full Names
RAB5C, member RAS oncogene family
NCBI Official Symbol
RAB5C
NCBI Official Synonym Symbols
RABL; L1880; RAB5L; RAB5CL
NCBI Protein Information
ras-related protein Rab-5C
UniProt Protein Name
Ras-related protein Rab-5C
Protein Family
UniProt Gene Name
RAB5C
UniProt Synonym Gene Names
RABL
UniProt Entry Name
RAB5C_HUMAN
NCBI Description
Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]).[supplied by OMIM, Nov 2010]
Uniprot Description
RAB5C: Protein transport. Probably involved in vesicular traffic. Belongs to the small GTPase superfamily. Rab family.
Protein type: G protein, monomeric, Rab; Motility/polarity/chemotaxis; G protein; G protein, monomeric
Chromosomal Location of Human Ortholog: 17q21.2
Cellular Component: intracellular membrane-bound organelle; lysosomal membrane; endocytic vesicle; early endosome membrane; early endosome; plasma membrane; melanosome; lipid particle
Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding
Biological Process: intracellular protein transport; metabolic process; regulation of endocytosis; plasma membrane to endosome transport; Rab protein signal transduction
Research Articles on RAB5C
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Product Notes
The Human RAB5C rab5c (Catalog #AAA9714653) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714653 ELISA Kit recognizes Human RAB5C. It is sometimes possible for the material contained within the vial of "Ras-Related Protein Rab-5C (RAB5C), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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