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Typical Testing Data/Standard Curve (for reference only)

Human L-amino-acid oxidase, IL4I1 ELISA Kit | IL4I1 elisa kit

Human L-amino-acid oxidase, IL4I1 ELISA Kit

Gene Names
IL4I1; LAO; FIG1; LAAO
Reactivity
Human
Synonyms
L-amino-acid oxidase; IL4I1; Human L-amino-acid oxidase; IL4I1 ELISA Kit; IL4I1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
567
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.05-15ng/ml
Sensitivity
0.02ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL4I1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human L-amino-acid oxidase (also known as IL4I1) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human IL4I1 antibody. IL4I1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL4I1 Antibody is added and binds to IL4I1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL4I1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL4I1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
L-amino-acid oxidase isoform 1
NCBI Official Synonym Full Names
interleukin 4 induced 1
NCBI Official Symbol
IL4I1
NCBI Official Synonym Symbols
LAO; FIG1; LAAO
NCBI Protein Information
L-amino-acid oxidase
UniProt Protein Name
L-amino-acid oxidase
UniProt Gene Name
IL4I1
UniProt Synonym Gene Names
FIG1; LAAO; LAO; IL4-induced protein 1; hFIG1
UniProt Entry Name
OXLA_HUMAN

NCBI Description

This gene encodes a protein with limited similarity to L-amino acid oxidase which contains the conserved amino acids thought to be involved in catalysis and binding of flavin adenine dinucleotide (FAD) cofactor. The expression of this gene can be induced by interleukin 4 in B cells, however, expression of transcripts containing the first two exons of the upstream gene is found in other cell types. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

IL4I1: Lysosomal L-amino-acid oxidase with highest specific activity with phenylalanine. May play a role in lysosomal antigen processing and presentation. Belongs to the flavin monoamine oxidase family. FIG1 subfamily. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Oxidoreductase; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 1.4.3.2; Secreted; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - tryptophan; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: lysosome; extracellular region

Molecular Function: L-amino-acid oxidase activity

Research Articles on IL4I1

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Product Notes

The Human IL4I1 il4i1 (Catalog #AAA1607621) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1607621 ELISA Kit recognizes Human IL4I1. It is sometimes possible for the material contained within the vial of "L-amino-acid oxidase, IL4I1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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