Western Blot (WB)
(Western Blot analysis of extracts from C2C12 cells untreated or treated with PMA using elF2beta (Phospho-Ser2) antibody)
Rabbit eIF2 beta Polyclonal Antibody | anti-eIF2 beta antibody
eIF2 beta antibody
Applications
Western Blot
Purity
eIF2 beta antibody was purified by immunoaffinity chromatography.
Synonyms
eIF2 beta; Polyclonal Antibody; eIF2 beta antibody; Polyclonal eIF2 beta; Anti-eIF2 beta; eIF beta-2; Eukaryotic Initiation Factor 2; Phospho Ser2; eIF beta 2; anti-eIF2 beta antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
eIF2 beta antibody was purified by immunoaffinity chromatography.
Form/Format
Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-eIF2 beta antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Biological Significance
eIF2 is a eukaryotic initiation factor. It is required in the initiation of translation. In this fundamental process of life, the ribosome builds proteins according to the information encoded on the mRNA. eIF2 mediates the binding of tRNAmet to the ribosome in a GTP-dependent manner. eIF2 is a heterotrimer consisting of an alpha (also called subunit 1), a beta (subunit 2), and a gamma (subunit 3) subunit. Once the initiation is completed, eIF2 is released from the ribosome bound to GDP as an inactive binary complex. To participate in another round of translation initiation, this GDP must be exchanged for GTP.
Cross-Reactivity
Human, Mouse
Immunogen
eIF2 beta antibody was raised in Rabbit using the peptide sequence around phosphorylation site of serine 2 (M-S(p)-G-D-E) derived from eIF2B as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts from C2C12 cells untreated or treated with PMA using elF2beta (Phospho-Ser2) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from C2C12 cells untreated or treated with PMA using elF2beta (Phospho-Ser2) antibody)
Related Product Information for anti-eIF2 beta antibody
Rabbit polyclonal eIF2 beta antibody
Product Categories/Family for anti-eIF2 beta antibody
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Product Notes
The eIF2 beta (Catalog #AAA837454) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's eIF2 beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the eIF2 beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF2 beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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