Human Interleukin 4 Induced Protein 1 (IL4I1) ELISA Kit | IL4I1 elisa kit

Human Interleukin 4 Induced Protein 1 (IL4I1) ELISA Kit

Cat. Num. AAA2023147

• Gene Names: IL4I1; LAO; FIG1; LAAO
• Reactivity: Human
• Synonyms: Interleukin 4 Induced Protein 1 (IL4I1); Human Interleukin 4 Induced Protein 1 (IL4I1) ELISA Kit; FIG1; LAAO; LAO; L-amino-acid oxidase; IL4I1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 4 Induced Protein 1 (IL4I1).; No significant cross-reactivity or interference between Interleukin 4 Induced Protein 1 (IL4I1) and analogues was observed.
• Sequence Length: 589

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 4 Induced Protein 1 (IL4I1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 4 Induced Protein 1 (IL4I1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Related Product Information for IL4I1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 4 Induced Protein 1 (IL4I1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 4 Induced Protein 1 (IL4I1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 4 Induced Protein 1 (IL4I1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 4 Induced Protein 1 (IL4I1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL4I1 elisa kit

Cytokine

NCBI and Uniprot Product Information

• NCBI GI #: 71060513
• NCBI GeneID: 259307
• UniProt Accession #: Q96RQ9; Q1WMJ3; Q4GZN1; Q4GZN2; Q6P2Q3; Q8TEM5; Q96RQ8
• Molecular Weight: 65,328 Da
• NCBI Official Full Name: interleukin 4 induced protein 1
• NCBI Official Synonym Full Names: interleukin 4 induced 1
• NCBI Official Symbol: IL4I1
• NCBI Official Synonym Symbols: LAO; FIG1; LAAO
• NCBI Protein Information: L-amino-acid oxidase
• UniProt Protein Name: L-amino-acid oxidase
• UniProt Gene Name: IL4I1
• UniProt Synonym Gene Names: FIG1; LAAO; LAO; IL4-induced protein 1; hFIG1
• UniProt Entry Name: OXLA_HUMAN

NCBI Description

This gene encodes a protein with limited similarity to L-amino acid oxidase which contains the conserved amino acids thought to be involved in catalysis and binding of flavin adenine dinucleotide (FAD) cofactor. The expression of this gene can be induced by interleukin 4 in B cells, however, expression of transcripts containing the first two exons of the upstream gene is found in other cell types. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

IL4I1: Lysosomal L-amino-acid oxidase with highest specific activity with phenylalanine. May play a role in lysosomal antigen processing and presentation. Belongs to the flavin monoamine oxidase family. FIG1 subfamily. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Secreted, signal peptide; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - tyrosine; EC 1.4.3.2; Secreted; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - cysteine and methionine; Oxidoreductase

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: lysosome; extracellular region

Molecular Function: L-amino-acid oxidase activity