Typical Testing Data/Standard Curve (for reference only)
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Human Anoctamin 2 (ANO2) ELISA Kit | ANO2 elisa kit
Human Anoctamin 2 (ANO2) ELISA Kit
Gene Names
ANO2; C12orf3; TMEM16B
Reactivity
Human
Synonyms
Anoctamin 2 (ANO2); Human Anoctamin 2 (ANO2) ELISA Kit; ANO2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of ANO2. No significant cross-reactivity or interference between ANO2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANO2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for ANO2 elisa kit
Intended Uses: This ANO2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ANO2. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: ANO2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ANO2 antibody and an ANO2-HRP conjugate. The assay sample and buffer are incubated together with ANO2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANO2 concentration since ANO2 from samples and ANO2-HRP conjugate compete for the anti-ANO2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANO2 from the sample, fewer sites are left to bind ANO2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANO2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ANO2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ANO2 antibody and an ANO2-HRP conjugate. The assay sample and buffer are incubated together with ANO2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANO2 concentration since ANO2 from samples and ANO2-HRP conjugate compete for the anti-ANO2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANO2 from the sample, fewer sites are left to bind ANO2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANO2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ANO2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
113,616 Da
NCBI Official Full Name
anoctamin-2 isoform 2
NCBI Official Synonym Full Names
anoctamin 2, calcium activated chloride channel
NCBI Official Symbol
ANO2
NCBI Official Synonym Symbols
C12orf3; TMEM16B
NCBI Protein Information
anoctamin-2; transmembrane protein 16B (eight membrane-spanning domains)
UniProt Protein Name
Anoctamin-2
Protein Family
UniProt Gene Name
ANO2
UniProt Synonym Gene Names
C12orf3; TMEM16B
UniProt Entry Name
ANO2_HUMAN
NCBI Description
ANO2 belongs to a family of calcium-activated chloride channels (CaCCs) (reviewed by Hartzell et al., 2009 [PubMed 19015192]).[supplied by OMIM, Jan 2011]
Uniprot Description
ANO2: Acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. May mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Transporter; Transporter, ion channel; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13.3
Cellular Component: nucleoplasm; nonmotile primary cilium; plasma membrane
Molecular Function: protein homodimerization activity; intracellular calcium activated chloride channel activity; protein heterodimerization activity
Biological Process: transmembrane transport
Research Articles on ANO2
Similar Products
Product Notes
The Human ANO2 ano2 (Catalog #AAA7225867) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7225867 ELISA Kit recognizes Human ANO2. It is sometimes possible for the material contained within the vial of "Anoctamin 2 (ANO2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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