Anoctamin 2 Recombinant Protein | ANO2 recombinant protein

Recombinant Anoctamin 2 (ANO2)

Cat. Num. AAA2034455

• Gene Names: ANO2; C12orf3; TMEM16B
• Reactivity: Homo sapiens (Human)
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Anoctamin 2; Recombinant Anoctamin 2 (ANO2); TMEM16B; C12orf3; Transmembrane Protein 16B(Eight Membrane-Spanning Domains); ANO2 recombinant protein

• Host: E.coli
• Reactivity: Homo sapiens (Human)
• Purity/Purification: > 90%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
• Concentration: Original Concentration: 200ug/mL (varies by lot)

• Applicable Applications for ANO2 recombinant protein: Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
• Source: Prokaryotic expression
• Residues: Ala823~Val1003
• Tags: N-terminal His Tag
• Subcellular Location: Membrane
• Traits: Freeze-dried powder
• Predicted isoelectric point: 5.9
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence

Gene Sequencing (extract)

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 515870521
• NCBI GeneID: 57101
• NCBI Accession #: NP_001265526.1
• NCBI GenBank Nucleotide #: NM_001278597.1
• Molecular Weight: Predicted Molecular Mass: 24.4kDa; Accurate Molecular Mass: 24kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: anoctamin-2 isoform 2
• NCBI Official Synonym Full Names: anoctamin 2
• NCBI Official Symbol: ANO2
• NCBI Official Synonym Symbols: C12orf3; TMEM16B
• NCBI Protein Information: anoctamin-2
• UniProt Protein Name: Anoctamin-2
• Protein Family: Anoctamin
• UniProt Gene Name: ANO2
• UniProt Synonym Gene Names: C12orf3; TMEM16B
• UniProt Entry Name: ANO2_HUMAN

NCBI Description

ANO2 belongs to a family of calcium-activated chloride channels (CaCCs) (reviewed by Hartzell et al., 2009 [PubMed 19015192]).[supplied by OMIM, Jan 2011]

Uniprot Description

ANO2: Acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. May mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, multi-pass; Transporter, ion channel; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: nonmotile primary cilium; nucleoplasm; plasma membrane

Molecular Function: intracellular calcium activated chloride channel activity; protein heterodimerization activity; protein homodimerization activity

Biological Process: transmembrane transport