Human Adenylate Cyclase Type 1 (ADCY1) ELISA Kit | ADCY1 elisa kit

Human Adenylate Cyclase Type 1 (ADCY1) ELISA Kit

Cat. Num. AAA9714593

• Gene Names: ADCY1; AC1; DFNB44
• Reactivity: Human
• Synonyms: Adenylate Cyclase Type 1 (ADCY1); Human Adenylate Cyclase Type 1 (ADCY1) ELISA Kit; ADCY1; AC1; 3; 5-cyclic AMP synthetase; ATP pyrophosphate-lyase; Ca(2+)/calmodulin-activated adenylyl cyclase; adenyl cyclase; adenylate cyclase 1; adenylate cyclase type I; ADCY1 elisa kit

• Reactivity: Human
• Specificity: Human Adenylate cyclase type 1 (ADCY1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADCY1. No significant cross-reactivity or interference between Human ADCY1 and analogues was observed.
• Sequence Length: 1119

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Adenylate cyclase type 1 (ADCY1) Standard Curve.)

Related Product Information for ADCY1 elisa kit

Description: This Human Adenylate cyclase type 1 (ADCY1) ELISA Kit employs a two-site sandwich ELISA to quantitate ADCY1 in samples. An antibody specific for ADCY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADCY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADCY1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADCY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Adenylate cyclase type 1 (ADCY1) encodes a member of the of adenylate cyclase gene family that is primarily expressed in the brain. Adenylate cyclase 1 is regulated by calcium/calmodulin concentration and may be involved in brain development. Alternate splicing results in multiple transcript variants. 

NCBI and Uniprot Product Information

• NCBI GI #: 31083193
• NCBI GeneID: 107
• NCBI Accession #: NP_066939.1
• NCBI GenBank Nucleotide #: NP_066939.1
• UniProt Accession #: Q08828
• NCBI Official Full Name: adenylate cyclase type 1 isoform 1
• NCBI Official Synonym Full Names: adenylate cyclase 1
• NCBI Official Symbol: ADCY1
• NCBI Official Synonym Symbols: AC1; DFNB44
• NCBI Protein Information: adenylate cyclase type 1
• UniProt Protein Name: Adenylate cyclase type 1
• Protein Family: Adenylate cyclase
• UniProt Gene Name: ADCY1

NCBI Description

This gene encodes a member of the of adenylate cyclase gene family that is primarily expressed in the brain. This protein is regulated by calcium/calmodulin concentration and may be involved in brain development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling. Mediates responses to increased cellular Ca2+/calmodulin levels (). May be involved in regulatory processes in the central nervous system. May play a role in memory and learning. Plays a role in the regulation of the circadian rhythm of daytime contrast sensitivity probably by modulating the rhythmic synthesis of cyclic AMP in the retina ().