Human Adenylate cyclase type 1 ELISA Kit | ADCY1 elisa kit

Human Adenylate cyclase type 1 ELISA Kit

Cat. Num. AAA760525

• Gene Names: ADCY1; AC1; DFNB44
• Reactivity: Human
• Synonyms: Adenylate cyclase type 1; Human Adenylate cyclase type 1 ELISA Kit; ADCY1/Adenylate cyclase type 1/Ca (2+); calmodulin-activated adenylyl cyclase/Adenylyl cyclase 1/ATP pyrophosphate-lyase 1/Adenylate cyclase type I; ADCY1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ADCY1 . No significant cross-reactivity or interference between ADCY1 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between ADCY1 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 1119

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/ml
• Sensitivity: <0.469ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADCY1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADCY1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADCY1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ADCY1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ADCY1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ADCY1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ADCY1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 31083193
• NCBI GeneID: 107
• NCBI Accession #: NP_066939.1
• NCBI GenBank Nucleotide #: NM_021116.2
• Molecular Weight: 123,440 Da
• NCBI Official Full Name: adenylate cyclase type 1 isoform 1
• NCBI Official Synonym Full Names: adenylate cyclase 1 (brain)
• NCBI Official Symbol: ADCY1
• NCBI Official Synonym Symbols: AC1; DFNB44
• NCBI Protein Information: adenylate cyclase type 1
• UniProt Protein Name: Adenylate cyclase type 1
• Protein Family: Adenylate cyclase
• UniProt Gene Name: ADCY1
• UniProt Entry Name: ADCY1_HUMAN

NCBI Description

This gene encodes a member of the of adenylate cyclase gene family that is primarily expressed in the brain. This protein is regulated by calcium/calmodulin concentration and may be involved in brain development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

ADCY1: a calmodulin-sensitive adenylyl cyclase expressed in brain. The mouse protein is involved in pattern formation of the brain. May be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning.

Protein type: Adenylyl cyclase; Transporter; Membrane protein, multi-pass; Lyase; EC 4.6.1.1; Transporter, aquaporin family; Nucleotide Metabolism - purine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: cytoplasm; integral to plasma membrane; lipid raft; nucleus; plasma membrane

Molecular Function: adenylate cyclase activity; ATP binding; calcium- and calmodulin-responsive adenylate cyclase activity; calmodulin binding; metal ion binding

Biological Process: activation of protein kinase A; axonogenesis; cAMP biosynthetic process; cAMP-mediated signaling; circadian rhythm; energy reserve metabolic process; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; G-protein signaling, adenylate cyclase activating pathway; G-protein signaling, adenylate cyclase inhibiting pathway; innate immune response; long-term memory; nerve growth factor receptor signaling pathway; phospholipase C activation; regulation of circadian rhythm; renal water homeostasis; response to drug; response to lithium ion; signal transduction; synaptic transmission; transmembrane transport; water transport

Disease: Deafness, Autosomal Recessive 44