Western Blot (WB)
(Western blot analysis of PXN(arrow) using rabbit polyclonal PXN Antibody (Y118). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with PXN gene (Lane 2) (Origene Technologies).)
Rabbit anti-Human PXN Polyclonal Antibody | anti-PXN antibody
PXN Antibody (Y118)
Western Blot (WB)
(Western blot analysis of PXN(arrow) using rabbit polyclonal PXN Antibody (Y118). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with PXN gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of PXN(arrow) using rabbit polyclonal PXN Antibody (Y118). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with PXN gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PXN Antibody (Y118) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PXN Antibody (Y118) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]
Uniprot Description
PXN: a multi-domain cytoskeletal protein involved in actin-membrane attachment that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions. Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the Crk SH2 domain. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.Three alternatively-spliced isoforms have been described. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase.
Protein type: Cell adhesion; Adaptor/scaffold; Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 12q24.31
Cellular Component: nucleoplasm; microtubule associated complex; focal adhesion; lamellipodium; cytoplasm; stress fiber; plasma membrane; cell cortex; cytosol
Molecular Function: integrin binding; protein binding; zinc ion binding; beta-catenin binding; BH4 domain binding; protein kinase binding; vinculin binding
Biological Process: integrin-mediated signaling pathway; focal adhesion formation; epidermal growth factor receptor signaling pathway; lamellipodium biogenesis; peptidyl-tyrosine phosphorylation; activation of MAPK activity; signal complex assembly; cytoskeleton organization and biogenesis; signal transduction; regulation of cell shape; branching morphogenesis of a tube; muscle contraction; transforming growth factor beta receptor signaling pathway; cell adhesion; vascular endothelial growth factor receptor signaling pathway
Research Articles on PXN
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Product Notes
The PXN pxn (Catalog #AAA9208703) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 94-125. The PXN Antibody (Y118) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PXN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the PXN pxn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PXN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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