Human Adenylate Cyclase 1, Brain ELISA Kit | ADCY1 elisa kit

Human Adenylate Cyclase 1, Brain ELISA Kit

Cat. Num. AAA730718

• Gene Names: ADCY1; AC1; DFNB44
• Reactivity: Human
• Synonyms: Adenylate Cyclase 1; Brain; Human Adenylate Cyclase 1; Brain ELISA Kit; ADCY1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ADCY1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADCY1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADCY1 elisa kit

Principle of the Assay: ADCY1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADCY1 antibody and an ADCY1-HRP conjugate. The assay sample and buffer are incubated together with ADCY1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADCY1 concentration since ADCY1 from samples and ADCY1-HRP conjugate compete for the anti-ADCY1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADCY1 from the sample, fewer sites are left to bind ADCY1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADCY1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ADCY1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 31083193
• NCBI GeneID: 107
• NCBI Accession #: NP_066939.1
• NCBI GenBank Nucleotide #: NM_021116.2
• Molecular Weight: 123,440 Da
• NCBI Official Full Name: adenylate cyclase type 1 isoform 1
• NCBI Official Synonym Full Names: adenylate cyclase 1 (brain)
• NCBI Official Symbol: ADCY1
• NCBI Official Synonym Symbols: AC1; DFNB44
• NCBI Protein Information: adenylate cyclase type 1; adenyl cyclase; adenylyl cyclase 1; adenylate cyclase type I; ATP pyrophosphate-lyase 1; 3',5'-cyclic AMP synthetase; Ca(2+)/calmodulin-activated adenylyl cyclase
• UniProt Protein Name: Adenylate cyclase type 1
• Protein Family: Adenylate cyclase
• UniProt Gene Name: ADCY1
• UniProt Entry Name: ADCY1_HUMAN

NCBI Description

This gene encodes a member of the of adenylate cyclase gene family that is primarily expressed in the brain. This protein is regulated by calcium/calmodulin concentration and may be involved in brain development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

ADCY1: a calmodulin-sensitive adenylyl cyclase expressed in brain. The mouse protein is involved in pattern formation of the brain. May be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning.

Protein type: Membrane protein, integral; Transporter, aquaporin family; Nucleotide Metabolism - purine; Transporter; Adenylyl cyclase; EC 4.6.1.1; Membrane protein, multi-pass; Lyase

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: cytoplasm; plasma membrane; integral to membrane; nucleus

Molecular Function: calmodulin binding; metal ion binding; calcium- and calmodulin-responsive adenylate cyclase activity; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; response to drug; circadian rhythm; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; response to lithium ion; water transport; activation of protein kinase A; signal transduction; regulation of circadian rhythm; G-protein signaling, adenylate cyclase activating pathway; synaptic transmission; long-term memory; axonogenesis; phospholipase C activation; energy reserve metabolic process; renal water homeostasis; innate immune response; cAMP biosynthetic process; G-protein signaling, adenylate cyclase inhibiting pathway; transmembrane transport

Disease: Deafness, Autosomal Recessive 44