Human Adenylate Cyclase 1, Brain (ADCY1) ELISA Kit | ADCY1 elisa kit

Human Adenylate Cyclase 1, Brain (ADCY1) ELISA Kit

Cat. Num. AAA2020221

• Gene Names: ADCY1; AC1; DFNB44
• Reactivity: Human
• Synonyms: Adenylate Cyclase 1; Brain (ADCY1); Human Adenylate Cyclase 1; Brain (ADCY1) ELISA Kit; AC1; ATP pyrophosphate-lyase 1; Ca(2+)/calmodulin-activated adenylyl cyclase; Adenylate cyclase type I; ADCY1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adenylate Cyclase 1, Brain (ADCY1).; No significant cross-reactivity or interference between Adenylate Cyclase 1, Brain (ADCY1) and analogues was observed.
• Sequence Length: 1119

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.78-50ng/mL
• Sensitivity: < 0.29ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Cyclase 1, Brain (ADCY1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Cyclase 1, Brain (ADCY1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADCY1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase 1, Brain (ADCY1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase 1, Brain (ADCY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase 1, Brain (ADCY1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenylate Cyclase 1, Brain (ADCY1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ADCY1 elisa kit

Enzyme & Kinase; Metabolic pathway; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 31083193
• NCBI GeneID: 107
• NCBI Accession #: NP_066939.1
• NCBI GenBank Nucleotide #: NM_021116.2
• UniProt Accession #: Q08828; Q75MI1; A4D2L8
• Molecular Weight: 123,440 Da
• NCBI Official Full Name: adenylate cyclase type 1 isoform 1
• NCBI Official Synonym Full Names: adenylate cyclase 1 (brain)
• NCBI Official Symbol: ADCY1
• NCBI Official Synonym Symbols: AC1; DFNB44
• NCBI Protein Information: adenylate cyclase type 1
• UniProt Protein Name: Adenylate cyclase type 1
• Protein Family: Adenylate cyclase
• UniProt Gene Name: ADCY1
• UniProt Entry Name: ADCY1_HUMAN

NCBI Description

This gene encodes a member of the of adenylate cyclase gene family that is primarily expressed in the brain. This protein is regulated by calcium/calmodulin concentration and may be involved in brain development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

ADCY1: a calmodulin-sensitive adenylyl cyclase expressed in brain. The mouse protein is involved in pattern formation of the brain. May be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning.

Protein type: Membrane protein, multi-pass; Nucleotide Metabolism - purine; Lyase; Transporter, aquaporin family; Adenylyl cyclase; Membrane protein, integral; EC 4.6.1.1; Transporter

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: cytoplasm; integral to membrane; plasma membrane; nucleus

Molecular Function: calmodulin binding; metal ion binding; calcium- and calmodulin-responsive adenylate cyclase activity; ATP binding

Biological Process: circadian rhythm; response to drug; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; response to lithium ion; activation of protein kinase A; signal transduction; regulation of circadian rhythm; G-protein signaling, adenylate cyclase activating pathway; synaptic transmission; long-term memory; axonogenesis; phospholipase C activation; energy reserve metabolic process; renal water homeostasis; innate immune response; cAMP biosynthetic process; G-protein signaling, adenylate cyclase inhibiting pathway; transmembrane transport

Disease: Deafness, Autosomal Recessive 44