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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Rat Adenylyl cyclase 1 (AC-1) Standard Curve.)

Rat Adenylyl Cyclase 1 (AC-1) ELISA Kit | AC-1 elisa kit

Rat Adenylyl Cyclase 1 (AC-1) ELISA Kit

Gene Names
Dusp2; PAC1
Reactivity
Rat
Synonyms
Adenylyl Cyclase 1 (AC-1); Rat Adenylyl Cyclase 1 (AC-1) ELISA Kit; AC-1; AC1; 3; 5-cyclic AMP synthetase; ATP pyrophosphate-lyase; Ca(2+)/calmodulin-activated adenylyl cyclase; adenyl cyclase; adenylate cyclase 1; adenylate cyclase type I; AC-1 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
Rat Adenylyl cyclase 1 (AC-1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat AC-1. No significant cross-reactivity or interference between Rat AC-1 and analogues was observed.
Sequence Length
318
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat Adenylyl cyclase 1 (AC-1) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Rat Adenylyl cyclase 1 (AC-1) Standard Curve.)
Related Product Information for AC-1 elisa kit
Description: This Rat Adenylyl cyclase 1 (AC-1) ELISA Kit employs a two-site sandwich ELISA to quantitate AC-1 in samples. An antibody specific for AC-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AC-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AC-1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AC-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: AC-1 gene encodes a member of the of adenylate cyclase gene family that is primarily expressed in the brain. This protein is regulated by calcium/calmodulin concentration and may be involved in brain development.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dual specificity protein phosphatase 2
NCBI Official Synonym Full Names
dual specificity phosphatase 2
NCBI Official Symbol
Dusp2
NCBI Official Synonym Symbols
PAC1
NCBI Protein Information
dual specificity protein phosphatase 2
UniProt Protein Name
Dual specificity protein phosphatase 2
UniProt Gene Name
Dusp2
UniProt Synonym Gene Names
Pac-1; Pac1

Uniprot Description

DUSP2: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP2 is induced by mitogens, inactivates ERK1 and ERK2, is predominantly expressed in hematopoietic tissues, and is localized in the nucleus. Contains 1 rhodanese domain.

Protein type: EC 3.1.3.16; EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 2|2 F1

Cellular Component: nuclear membrane; nucleus

Molecular Function: hydrolase activity; MAP kinase tyrosine/serine/threonine phosphatase activity; mitogen-activated protein kinase binding; phosphoprotein phosphatase activity; phosphoric monoester hydrolase activity; protein binding; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity

Biological Process: dephosphorylation; endoderm formation; protein amino acid dephosphorylation

Research Articles on AC-1

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Product Notes

The Rat AC-1 dusp2 (Catalog #AAA9711944) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711944 ELISA Kit recognizes Rat AC-1. It is sometimes possible for the material contained within the vial of "Adenylyl Cyclase 1 (AC-1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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