Human MRPL53 (Mitochondrial large ribosomal subunit protein mL53) ELISA Kit | MRPL53 elisa kit

Human MRPL53 (Mitochondrial large ribosomal subunit protein mL53) ELISA Kit

Cat. Num. AAA7608115

• Gene Names: MRPL53; L53MT
• Reactivity: Human
• Synonyms: MRPL53 (Mitochondrial large ribosomal subunit protein mL53); Human MRPL53 (Mitochondrial large ribosomal subunit protein mL53) ELISA Kit; 39S ribosomal protein L53; mitochondrial; L53mt; MRP-L53; Mitochondrial large ribosomal subunit protein mL53; MRPL53 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MRPL53. No significant cross-reactivity or interference between MRPL53 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MRPL53 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 16596694
• NCBI GeneID: 116540
• NCBI Accession #: NP_444278.1
• NCBI GenBank Nucleotide #: NM_053050.4
• UniProt Accession #: Q96EL3
• Molecular Weight: 12,107 Da
• NCBI Official Full Name: 39S ribosomal protein L53, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L53
• NCBI Official Symbol: MRPL53
• NCBI Official Synonym Symbols: L53MT
• NCBI Protein Information: 39S ribosomal protein L53, mitochondrial
• UniProt Protein Name: 39S ribosomal protein L53, mitochondrial
• Protein Family: 39S ribosomal protein
• UniProt Gene Name: MRPL53
• UniProt Synonym Gene Names: L53mt; MRP-L53
• UniProt Entry Name: RM53_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 1p. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPL53:

Protein type: Ribosomal; Mitochondrial

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: mitochondrial inner membrane; ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis