Human CD163/M130 ELISA Kit | CD163/M130 elisa kit

Human CD163/M130 PicoKine Fast ELISA Kit

Cat. Num. AAA1752744

• Gene Names: CD163; M130; MM130; SCARI1
• Reactivity: Human
• Synonyms: CD163/M130; Human CD163/M130 PicoKine Fast ELISA Kit; Human CD163; Scavenger receptor cysteine-rich type 1 protein M130; Hemoglobin scavenger receptor; CD163; Soluble CD163; sCD163; M130; CD163/M130 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CD163
• Sequence Length: 1156

• Samples: Cell culture supernates, serum and plasma(heparin)
• Detection Range: 1.56ng/ml-100ng/ml
• Sensitivity: <150pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD163/M130 elisa kit

Principle of the Assay: The Picokine™ Human CD163 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD163 with a 96-well strip plate that is pre-coated with antibody specific for CD163. The detection antibody is a biotinylated antibody specific for CD163. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CD163 with immunogen: Expression system for standard: NSO; Immunogen sequence: G41-S1045. The kit is analytically validated with ready to use reagents. To measure Human CD163, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human CD163 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD163 in the sample.!!Background/Introduction: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.

NCBI and Uniprot Product Information

• NCBI GI #: 344179110
• NCBI GeneID: 9332
• NCBI Accession #: NP_004235.4
• NCBI GenBank Nucleotide #: NP_004235.4
• UniProt Accession #: Q86VB7
• NCBI Official Full Name: scavenger receptor cysteine-rich type 1 protein M130 isoform a
• NCBI Official Synonym Full Names: CD163 molecule
• NCBI Official Symbol: CD163
• NCBI Official Synonym Symbols: M130; MM130; SCARI1
• NCBI Protein Information: scavenger receptor cysteine-rich type 1 protein M130
• UniProt Protein Name: Scavenger receptor cysteine-rich type 1 protein M130
• UniProt Gene Name: CD163
• UniProt Synonym Gene Names: M130; sCD163
• UniProt Entry Name: C163A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: protein binding; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; acute-phase response