SEMA3A cell lysate
Mouse SEMA3A HEK293 Overexpression Lysate
Gene Names
Sema3a; SemD; SEMA1; Semad; coll-1; Hsema-I
Applications
Western Blot
Synonyms
SEMA3A; Mouse SEMA3A HEK293 Overexpression Lysate; Mouse Semaphorin 3A/SEMA3A HEK293 Cell Lysate (WB positive control); sema domain; immunoglobulin domain (Ig); short basic domain; secreted; (semaphorin) 3A; Mouse coll-1 Overexpression Lysate; Mouse Hsema-I Overexpression Lysate; Mouse SEMA1 Overexpression Lysate; Mouse Semad Overexpression Lysate; Mouse SemD Overexpression Lysate; SEMA3A cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
781aa
Sequence
Lys26-Phe546
Applicable Applications for SEMA3A cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the N-terminal fragment (Lys 26-Phe 546) of mouse SEMA3A (O08665) was fused with the Fc region of human IgG1 at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SEMA3A cell lysate
Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 5 aa Sema domain. They are best characterized in relation to axon guidance during development of the nervous system. The functions of Semaphorins 3A (SEMA3A) are mediated primarily through binding to the Neuropilin-1 (Npn-1) and Plexin-A1 coreceptor complex. Neuropilins lack a signaling-competent cytoplasetmic domain and ensure semaphorin binding, whereas the transmembrane receptor plexin mediates the intracellular response. As the first identified vertebrate semaphorin, SEMA3A functions either as a chemorepulsive agent inhibiting axonal outgrowth, or as a chemoattractive agent stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Its overexpression is associated with schizophrenia which is seen in various human tumor cell lines, and aberrant release is associated with the progression of Alzheimer's disease
This Mouse SEMA3A overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SEMA3A protein from the overexpression lysate was verified.
This Mouse SEMA3A overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SEMA3A protein from the overexpression lysate was verified.
References
Giordano, A. et al., 2003, J Neurocytol.32(4):345-352. Good, P. F. et al., 2005, J. Neurochem.91(3): 716-736. Gu, C. et al., 2005, Science.307(5707): 265-268. Chadborn, N.H. et al., 2006, J Cell Sci.119(Pt 5):951-957. Schmidt, E.F. et al., 2007, Adv Exp Med Biol.600:1-11.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88,813 Da
NCBI Official Full Name
semaphorin-3A
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
NCBI Official Symbol
Sema3a
NCBI Official Synonym Symbols
SemD; SEMA1; Semad; coll-1; Hsema-I
NCBI Protein Information
semaphorin-3A; sema D; sema III; collapsin-1; semaphorin-D; semaphorin III
UniProt Protein Name
Semaphorin-3A
Protein Family
UniProt Gene Name
Sema3a
UniProt Synonym Gene Names
Semad; SemD; Sema III; Sema D
UniProt Entry Name
SEM3A_MOUSE
Uniprot Description
SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family.
Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis
Cellular Component: membrane; axon; dendrite; extracellular region
Molecular Function: chemorepellent activity; receptor activity; semaphorin receptor binding
Biological Process: trigeminal nerve structural organization; nervous system development; facial nerve structural organization; axon guidance; apoptosis; regulation of heart rate; olfactory bulb development; dendrite morphogenesis; multicellular organismal development; neuron migration; axonal fasciculation; negative chemotaxis; motor axon guidance; negative regulation of axon extension; negative regulation of axon extension involved in axon guidance; axon extension involved in axon guidance; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; branchiomotor neuron axon guidance; regulation of axon extension involved in axon guidance; nerve development; cell differentiation
Research Articles on SEMA3A
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Product Notes
The SEMA3A sema3a (Catalog #AAA8116749) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 781aa. AAA Biotech's SEMA3A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SEMA3A sema3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Lys26-Phe5 46. It is sometimes possible for the material contained within the vial of "SEMA3A, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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