Rabbit anti-Human SEMA3A Polyclonal Antibody | anti-SEMA3A antibody

Anti-SEMA3A Antibody, Rabbit Polyclonal

Cat. Num. AAA8108291

• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: SEMA3A; Polyclonal Antibody; Anti-SEMA3A Antibody; Rabbit Polyclonal; SEMA3A Antibody; Rabbit PAb; Antigen Affinity Purified; sema domain; immunoglobulin domain (Ig); short basic domain; secreted; (semaphorin) 3A; Anti-coll-1 Antibody; Anti-COLL1 Antibody; Anti-HH16 Antibody; Anti-Hsema-I Antibody; Anti-Hsema-III Antibody; Anti-SEMA1 Antibody; Anti-SEMAD Antibody; Anti-SEMAIII Antibody; Anti-SEMAL Antibody; Anti-Semaphorin 3A Antibody; Anti-SemD Antibody; anti-SEMA3A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SEMA3A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-SEMA3A antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:50-1:200; ICC/IF: 1:100-1:500
• Immunogen: E Coli-derived Human SEMA3A fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human SEMA3A fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human SEMA3A in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of SEMA3A in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SEMA3A polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Related Product Information for anti-SEMA3A antibody

Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 5 aa Sema domain. They are best characterized in relation to axon guidance during development of the nervous system. The functions of Semaphorins 3A (SEMA3A) are mediated primarily through binding to the Neuropilin-1 (Npn-1) and Plexin-A1 coreceptor complex. Neuropilins lack a signaling-competent cytoplasetmic domain and ensure semaphorin binding, whereas the transmembrane receptor plexin mediates the intracellular response. As the first identified vertebrate semaphorin, SEMA3A functions either as a chemorepulsive agent inhibiting axonal outgrowth, or as a chemoattractive agent stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Its overexpression is associated with schizophrenia which is seen in various human tumor cell lines, and aberrant release is associated with the progression of Alzheimer's disease

References

Giordano, A. et al., 2003, J Neurocytol.32(4):345-352. Good, P. F. et al., 2005, J. Neurochem.91(3): 716-736. Gu, C. et al., 2005, Science.307(5707): 265-268. Chadborn, N.H. et al., 2006, J Cell Sci.119(Pt 5):951-957. Schmidt, E.F. et al., 2007, Adv Exp Med Biol.600:1-11.