Semaphorin 3A (SEMA3A) Recombinant Protein | SEMA3A recombinant protein

Recombinant Semaphorin 3A (SEMA3A)

Cat. Num. AAA2010428

• Gene Names: Sema3a; SemD; SEMA1; Semad; coll-1; Hsema-I
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Semaphorin 3A (SEMA3A); Recombinant Semaphorin 3A (SEMA3A); SEMA3A recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-RLKLSYKEML ESNNVITFNG LANSSSYHTF LLDEERSRLY VGAKDHIFSF NLVNIKDFQK IVWPVSYTRR DECKWAGKDI LKECANFIKV LEAYNQTHLY ACGTGAFHPI CTYIEVGHHP
• Sequence Length: 772

• Applicable Applications for SEMA3A recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Arg31~Pro150 (Accession # O08665) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 340523100
• NCBI GeneID: 20346
• NCBI Accession #: NP_001230001.1
• NCBI GenBank Nucleotide #: NM_001243072.1
• UniProt Accession #: O08665; Q5BL08; Q62180; Q62215; E9QK85
• Molecular Weight: 17.7kDa
• NCBI Official Full Name: semaphorin-3A
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
• NCBI Official Symbol: Sema3a
• NCBI Official Synonym Symbols: SemD; SEMA1; Semad; coll-1; Hsema-I
• NCBI Protein Information: semaphorin-3A; sema D; sema III; collapsin-1; semaphorin-D; semaphorin III
• UniProt Protein Name: Semaphorin-3A
• Protein Family: Semaphorin
• UniProt Gene Name: Sema3a
• UniProt Synonym Gene Names: Semad; SemD; Sema III; Sema D
• UniProt Entry Name: SEM3A_MOUSE

Uniprot Description

SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Cellular Component: membrane; axon; dendrite; extracellular region

Molecular Function: chemorepellent activity; receptor activity; semaphorin receptor binding

Biological Process: trigeminal nerve structural organization; facial nerve structural organization; axon guidance; nervous system development; apoptosis; regulation of heart rate; multicellular organismal development; olfactory bulb development; dendrite morphogenesis; neuron migration; axonal fasciculation; negative chemotaxis; motor axon guidance; negative regulation of axon extension; negative regulation of axon extension involved in axon guidance; axon extension involved in axon guidance; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; branchiomotor neuron axon guidance; regulation of axon extension involved in axon guidance; nerve development; cell differentiation