SEMA3A recombinant protein

SEMA3A Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122878

• Gene Names: Sema3a; SemD; SEMA1; Semad; coll-1; Hsema-I
• Purity: >80% as determined by SDS-PAGE
• Synonyms: SEMA3A; SEMA3A Protein; Mouse; Recombinant (hFc Tag); Mouse Semaphorin 3A/SEMA3A Protein (Fc Tag); coll-1 Protein; Hsema-I Protein; SEMA1 Protein; Semad Protein; SemD Protein; sema domain; immunoglobulin domain (Ig); short basic domain; secreted; (semaphorin) 3A; SEMA3A recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Lys26-Phe546

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the N-terminal fragment (Lys 26-Phe 546) of mouse SEMA3A (O08665) was fused with the Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for SEMA3A recombinant protein

Background: Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 5 aa Sema domain. They are best characterized in relation to axon guidance during development of the nervous system. The functions of Semaphorins 3A (SEMA3A) are mediated primarily through binding to the Neuropilin-1 (Npn-1) and Plexin-A1 coreceptor complex. Neuropilins lack a signaling-competent cytoplasetmic domain and ensure semaphorin binding, whereas the transmembrane receptor plexin mediates the intracellular response. As the first identified vertebrate semaphorin, SEMA3A functions either as a chemorepulsive agent inhibiting axonal outgrowth, or as a chemoattractive agent stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Its overexpression is associated with schizophrenia which is seen in various human tumor cell lines, and aberrant release is associated with the progression of Alzheimer's disease

References

Giordano,A. et al., 2003, J Neurocytol.32(4):345-352. Good, P. F. et al., 2005, J. Neurochem.91(3): 716-736. Gu, C. et al., 2005, Science.307(5707): 265-268. Chadborn,N.H. et al., 2006, J Cell Sci.119(Pt 5):951-957. Schmidt,E.F. et al., 2007, Adv Exp Med Biol.600:1-11.

NCBI and Uniprot Product Information

• NCBI GI #: 340523100
• NCBI GeneID: 20346
• NCBI Accession #: NP_001230001.1
• NCBI GenBank Nucleotide #: NM_001243072.1
• UniProt Accession #: O08665; Q5BL08; Q62180; Q62215; E9QK85
• Molecular Weight: 88,813 Da
• NCBI Official Full Name: semaphorin-3A
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
• NCBI Official Symbol: Sema3a
• NCBI Official Synonym Symbols: SemD; SEMA1; Semad; coll-1; Hsema-I
• NCBI Protein Information: semaphorin-3A; sema D; sema III; collapsin-1; semaphorin-D; semaphorin III
• UniProt Protein Name: Semaphorin-3A
• Protein Family: Semaphorin
• UniProt Gene Name: Sema3a
• UniProt Synonym Gene Names: Semad; SemD; Sema III; Sema D
• UniProt Entry Name: SEM3A_MOUSE

Uniprot Description

SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Cellular Component: membrane; axon; dendrite; extracellular region

Molecular Function: chemorepellent activity; receptor activity; semaphorin receptor binding

Biological Process: trigeminal nerve structural organization; nervous system development; facial nerve structural organization; axon guidance; apoptosis; regulation of heart rate; olfactory bulb development; dendrite morphogenesis; multicellular organismal development; neuron migration; axonal fasciculation; negative chemotaxis; motor axon guidance; negative regulation of axon extension; negative regulation of axon extension involved in axon guidance; axon extension involved in axon guidance; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; branchiomotor neuron axon guidance; regulation of axon extension involved in axon guidance; nerve development; cell differentiation