SDS-PAGE
Semaphorin 3A / SEMA3A Recombinant Protein | SEMA3A recombinant protein
Recombinant Mouse Semaphorin 3A / SEMA3A Protein (Fc tag)
Gene Names
Sema3a; SemD; SEMA1; Semad; coll-1; Hsema-I
Purity
> 80 % as determined by SDS-PAGE
Synonyms
Semaphorin 3A / SEMA3A; Recombinant Mouse Semaphorin 3A / SEMA3A Protein (Fc tag); Hsema-I; SEMA1; SemD; Semad; coll-1; SEMA3A recombinant protein
Host
Human Cells
Purity/Purification
> 80 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
772
Application Notes
The recombinant mouse SEMA3A/Fc is a disulfide-linked homodimer The reduced monomer consists of 781 amino acids and has a predicted molecular mass of 87.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm SEMA3A/Fc monomer is approximately 100 kDa due to glycosylation.
Predicted N Terminal
Glu
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for SEMA3A recombinant protein
Background: Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 500 aa Sema domain. They are best characterized in relation to axon guidance during development of the nervous system. The functions of Semaphorins 3A (SEMA3A) are mediated primarily through binding to the Neuropilin-1 (Npn-1) and Plexin-A1 coreceptor complex. Neuropilins lack a signaling-competent cytoplasetmic domain and ensure semaphorin binding, whereas the transmembrane receptor plexin mediates the intracellular response. As the first identified vertebrate semaphorin, SEMA3A functions either as a chemorepulsive agent inhibiting axonal outgrowth, or as a chemoattractive agent stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Its overexpression is associated with schizophrenia which is seen in various human tumor cell lines, and aberrant release is associated with the progression of Alzheimer's disease
Description: A DNA sequence encoding the N-terminal fragment (Lys 26-Phe 546) of mouse SEMA3A (O08665) was fused with the Fc region of human IgG1 at the N-terminus.
Description: A DNA sequence encoding the N-terminal fragment (Lys 26-Phe 546) of mouse SEMA3A (O08665) was fused with the Fc region of human IgG1 at the N-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88,813 Da
NCBI Official Full Name
semaphorin-3A
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
NCBI Official Symbol
Sema3a
NCBI Official Synonym Symbols
SemD; SEMA1; Semad; coll-1; Hsema-I
NCBI Protein Information
semaphorin-3A
UniProt Protein Name
Semaphorin-3A
Protein Family
UniProt Gene Name
Sema3a
UniProt Synonym Gene Names
Semad; SemD; Sema III; Sema D
Uniprot Description
SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5|5 A1
Cellular Component: axon; dendrite; extracellular space
Molecular Function: chemorepellent activity; semaphorin receptor binding
Biological Process: apoptosis; axon extension involved in axon guidance; axon guidance; axonal fasciculation; branchiomotor neuron axon guidance; dendrite morphogenesis; facial nerve structural organization; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; motor axon guidance; negative chemotaxis; negative regulation of axon extension; negative regulation of axon extension involved in axon guidance; nerve development; neuron migration; olfactory bulb development; regulation of axon extension involved in axon guidance; regulation of heart rate; trigeminal nerve structural organization
Research Articles on SEMA3A
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Product Notes
The SEMA3A sema3a (Catalog #AAA2546876) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant mouse SEMA3A/Fc is a disulfide-linked homodimer The reduced monomer consists of 781 amino acids and has a predicted molecular mass of 87.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm SEMA3A/Fc monomer is approximately 100 kDa due to glycosylation. Researchers should empirically determine the suitability of the SEMA3A sema3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Semaphorin 3A / SEMA3A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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