OPCML active protein

OPCML Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123107

• Gene Names: Opcml; Gm181; Obcam; AI844366; 3732419F12; C230027C17; 2900075O15Rik; B930023M13Rik
• Purity: >95% as determined by SDS-PAGE
• Synonyms: OPCML; OPCML Protein; Mouse; Recombinant (His Tag); Mouse OBCAM/OPCML Protein (His Tag); 2900075O15Rik Protein; 3732419F12 Protein; AI844366 Protein; B930023M13Rik Protein; C230027C17 Protein; Gm181 Protein; Obcam Protein; opioid binding protein/cell adhesion molecule-like; OPCML active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala5-Val313

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Thr 30
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse OPCML (NP_808574.2)(Ala5-Val313) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized mouse OPCmL-His at 10ug/mL (100uL/well) can bind human LSAMP-Fc. The EC50 of human LSAMP-Fc is 0.04-0.1ug/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for OPCML active protein

Background: Opioid-binding Cell Adhesion Molecule (OBCAM), also known as OPCML, is a GPI-anchored cell adhesion molecule in the plasma membrane. This neuron-specific protein consists of three immunoglobulin (Ig)-like domains anchored to the membrane through a glycosylphosphatidylinositol (GPI)-tail. OPCML also belongs to the member of the IgLON family, a subgroup of the immunoglobulin superfamily, consisting of three members, LAMP, OBCAM, and Neurotrimin. These molecules interact homophilically and heterophilically within the family, and OBCAM acts only as heterodimers with LAMP or Neurotrimin and possibly inhibits neurite outgrowth from cerebellar granule cells. The OBCAM has been presumed to play a role as a cell adhesion/recognition molecule. Furthermore, the OPCML protein defects may play an important role in the carcinogenesis of cervical or ovarian cancers, and this gene is regarded as a candidate TSG (tumor suppressor gene).

References

Hachisuka A, et al. (2000) Developmental expression of opioid-binding cell adhesion molecule (OBCAM) in rat brain. Brain Res Dev Brain Res. 122(2): 183-91.Miyata S, et al. (2003) Polarized targeting of IgLON cell adhesion molecule OBCAM to dendrites in cultured neurons. Brain Res. 979(1-2): 129-36.Yamada M, et al. (2007) Synaptic adhesion molecule OBCAM; synaptogenesis and dynamic internalization. Brain Res. 1165: 5-14.Sugimoto C, et al. (2010) OBCAM, an immunoglobulin superfamily cell adhesion molecule, regulates morphology and proliferation of cerebral astrocytes. J Neurochem. 112(3): 818-28.

NCBI and Uniprot Product Information

• NCBI GI #: 51092272
• NCBI GeneID: 330908
• NCBI Accession #: NP_808574.2
• NCBI GenBank Nucleotide #: NM_177906.4
• NCBI Official Full Name: opioid-binding protein/cell adhesion molecule
• NCBI Official Synonym Full Names: opioid binding protein/cell adhesion molecule-like
• NCBI Official Symbol: Opcml
• NCBI Official Synonym Symbols: Gm181; Obcam; AI844366; 3732419F12; C230027C17; 2900075O15Rik; B930023M13Rik
• NCBI Protein Information: opioid-binding protein/cell adhesion molecule
• Protein Family: Opioid-binding protein/cell adhesion molecule